Alterations in hypertrophic gene expression by dietary copper restriction in mouse heart.
Alterations in hypertrophic gene expression by dietary copper restriction in mouse heart.
复制标题
饮食铜限制对小鼠心脏肥大基因表达的改变。
DOI：
10.1046/j.1525-1373.2000.22340.x
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Saari,JT
中科院分区：
文献类型：
--
作者：
Kang,YJ;Wu,H;Saari,JT
Dietary copper (Cu) restriction causes a hypertrophic cardiomyopathy similar to that induced by work overload in rodent models. However, a possible change in the program of hypertrophic gene expression has not been studied in the Cu-deficient heart. This study was undertaken to fill that gap. Dams of mouse pups were fed a Cu-deficient diet (0.35 mg/kg diet) or a Cu-adequate control diet (6.10 mg/kg) on the fourth day after birth, and weanling mice continued on the dams' diet until they were sacrificed. After 5 weeks of feeding, Cu concentrations were dramatically decreased in the heart and the liver of the mice fed the Cu-deficient diet. Corresponding to these changes, serum ceruloplasmin concentrations and hepatic Cu, Zn-superoxide dismutase activities were significantly (P < 0.05) depressed. The size of the Cu-deficient hearts was greatly enlarged as estimated from the absolute heart weight and the ratio of heart weight to body weight. The abundances of mRNAs for atrial natriuretic factor, β-myosin heavy chain, and α-skeletal actin in left ventricles were all significantly increased in the Cu- deficient hearts. Furthermore, Cu deficiency activated the expression of the c-myc oncogene in the left ventricle. This study thus demonstrated that a molecular program of alterations in embryonic genes, similar to that shown in the work-overloaded heart, was activated in the hypertrophied heart induced by Cu deficiency.
