Anti-HLA antibodies of predetermined specificity: a chemically synthesized peptide induces antibodies specific for HLA-A,B heavy chain.
Anti-HLA antibodies of predetermined specificity: a chemically synthesized peptide induces antibodies specific for HLA-A,B heavy chain.
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具有预定特异性的抗 HLA 抗体：化学合成的肽可诱导针对 HLA-A、B 重链的特异性抗体。
DOI：
10.1073/pnas.80.1.255
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发表时间：
1983
影响因子：
11.1
通讯作者：
Reisfeld,RA
中科院分区：
文献类型：
--
作者：
Church,WR;Walker,LE;Houghten,RA;Reisfeld,RA
化学合成的十二肽（Asp-Ala-Ala-Ser-Pro-Arg-Glu-Glu-Pro-Arg-Ala-Pro）由HLA-B7重链序列的第39至50位残基组成，并含有高度亲水性的六肽片段（Pro-Arg-Glu-Glu-Pro-Arg），诱导特异性结合游离HLA-A和HLA-B重链以及可溶性十二肽的抗体。尽管这些抗体在硝酸纤维素上的印迹中与游离HLA-A、B重链反应，但它们不能免疫沉淀通过非离子去污剂从人淋巴母细胞样细胞溶解的HLA-A、B-β 2-微球蛋白抗原复合物。这些观察结果表明，这种特殊的可溶性亲水性十二肽的物理特性可能与游离HLA-A、B重链表达的非同种异型决定簇非常相似，并且这些抗原位点可能被天然HLA-A、B重链-β 2-微球蛋白复合物中的构象变化所修饰。
A chemically synthesized dodecapeptide (Asp-Ala-Ala-Ser-Pro-Arg-Glu-Glu-Pro-Arg-Ala-Pro), consisting of residues 39 through 50 of the HLA-B7 heavy chain sequence and containing a highly hydrophilic hexapeptide segment (Pro-Arg-Glu-Glu-Pro-Arg), induced antibodies that bound specifically to free HLA-A, and HLA-B heavy chains and to the soluble dodecapeptide. Although these antibodies reacted with free HLA-A,B heavy chains in blots on nitrocellulose, they failed to immunoprecipitate the HLA-A,B-beta 2-microglobulin antigen complex solubilized from human lymphoblastoid cells by nonionic detergents. These observations suggest that the physical characteristics of this particular soluble hydrophilic dodecapeptide may closely resemble nonallotypic determinants expressed by free HLA-A,B heavy chains and that such antigenic sites may be modified by conformational changes in the native HLA-A,B heavy chain-beta 2-microglobulin complex.