ZP8, a neuronal zinc sensor with improved dynamic range; Imaging zinc in hippocampal slices with two-photon microscopy
ZP8, a neuronal zinc sensor with improved dynamic range; Imaging zinc in hippocampal slices with two-photon microscopy
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DOI：
10.1021/ic049293d
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发表时间：
2004-10-18
影响因子：
4.6
通讯作者：
Lippard, SJ
中科院分区：
文献类型：
--
作者：
Chang, CJ;Nolan, EM;Lippard, SJ
介绍了二氟荧光素单碳醛平台的合成及其在Zinpyr神经元Zn2+传感器家族新成员ZP8的制备中的应用。通过结合苯胺光诱导电子转移（PET）开关和吸电子荧光素支架，与之前的ZP探针相比，ZP8显示出更低的背景荧光和更高的动态范围。在Zn2+络合（Phi = 0.03-0.35）后，该明亮传感器的荧光强度增加了11倍，对Ca2+和Mg2+的细胞浓度具有高选择性。此外，ZP家族的传感器已被用于生物样品的双光子显微镜（TPM）光学成像，细胞渗透性ZP3探针能够利用TPM识别活海马片苔藓纤维突触内的天然不稳定Zn2+池，建立了该技术在监测内源性Zn2+储存方面的应用。
The synthesis of a difluorofluorescein monocarboxalclehyde platform and its use for preparing ZP8, a new member of the Zinpyr family of neuronal Zn2+ sensors, are described. By combining an aniline photoinduced electron transfer (PET) switch and an electron-withdrawing fluorescein scaffold, ZP8 displays reduced background fluorescence and improved dynamic range compared to previous ZP probes. The bright sensor undergoes an 11-fold increase in fluorescence intensity upon Zn2+ complexation (Phi = 0.03-0.35) with high selectivity over cellular concentrations of Ca2+ and Mg2+. In addition, sensors in the ZP family have been utilized for optical imaging in biological samples using two-photon microscopy (TPM), The cell-permeable ZP3 probe is capable of identifying natural pools of labile Zn2+ within the mossy fiber synapses of live hippocampal slices using TPM, establishing the application of this technique for monitoring endogenous Zn2+ stores.