Blood cell counter in gravity-driven microchannel
Blood cell counter in gravity-driven microchannel
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DOI：
10.1143/jjap.44.8739
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发表时间：
2005-12-01
期刊：
影响因子：
--
通讯作者：
Terada, N
中科院分区：
文献类型：
--
作者：
Yamada, H;Yoshida, Y;Terada, N
采用紫外激光烧蚀的方法在树脂中制备了三叉微通道。许多热硬化树脂薄膜被分层在苏打杯上。激光在每一层膜上制造一部分通道。河道深度45亩，宽度50-150亩。用激光在层压板上打通孔。带储液器的进水管插入孔中。一个血细胞可以在重力作用下运输。血液被引入通道的中心，从两侧通道的鞘流溶液。然后通过控制血库和溶液库之间的高度差将血细胞集中在连接处。
A three-pronged microchannel is fabricated in resin by UV laser ablation. A number of heat-hardened resin films are layered on a soda glass. A laser fabricates a part of the channel on each film for every lamination. The channels are 45 mu m in depth and 50-150 mu m in width. Through holes are made in the laminate film with a laser. Inlet pipes with reservoirs are inserted into the holes. A blood cell can be transported by gravity applied to it. The blood is led into the center of the channel with a sheath flow of solution from both side channels. Blood cells are then focused at the junction by controlling the height difference between a blood reservoir and the solution reservoirs.