Fructus mume Protects Against Cigarette Smoke Induced Chronic Cough Guinea Pig
Fructus mume Protects Against Cigarette Smoke Induced Chronic Cough Guinea Pig
复制标题
乌梅可预防香烟烟雾引起的豚鼠慢性咳嗽
DOI：
10.1089/jmf.2019.4423
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发表时间：
2020-02-01
影响因子：
2.4
通讯作者：
Lai, Kefang
中科院分区：
文献类型：
--
作者：
Xiang, Juan;Liu, Xiaodong;Lai, Kefang
乌梅治疗慢性咳嗽见于《中国药典》和中医典籍，但其疗效及相关成分尚不清楚。因此，我们研究了乌梅对香烟烟雾(CS)所致豚鼠慢性咳嗽的保护作用及其相关成分。采用高效液相色谱、凝胶渗透色谱、气相色谱-质谱法对梅花中的有机酸和多糖进行了检测。在CS染毒14天期间，给豚鼠灌胃赋形剂或乌梅水提物(FW)。用Buxco系统自动测量柠檬酸引起的咳嗽。苏木精-伊红染色观察肺组织病理改变及支气管肺泡灌洗液(BALF)中细胞分化情况。采用酶联免疫吸附试验检测肺组织中肿瘤坏死因子-α(TNF-α)和白介素8(IL-8)水平。用Alcian蓝-高碘酸席夫染色测定气管粘液产量。结果表明，梅花中柠檬酸、绿原酸和新绿原酸的含量较高，半乳糖和葡萄糖的比例较高，多糖的相对分子质量较低。与CS染毒组比较，FW组大鼠咳嗽次数明显减少，BALF和肺组织炎症细胞减少，气道上皮和粘膜下层增厚减轻。此外，FW还能明显抑制CS诱导的豚鼠肺组织和中心气道粘液中TNF-α和IL-8的过度产生。该提取物还可以通过减少咳嗽、呼吸道炎症和粘液过度产生来预防CS暴露引起的豚鼠慢性咳嗽。
Fructus mume was recorded in the Chinese Pharmacopoeia and traditional Chinese medical books for chronic cough, but the effect and related constituents are still unknown. Thus, we investigated the protect effects and the relevant constituents of F. mume in a guinea pig model with chronic cough induced by cigarette smoke (CS). The organic acids and polysaccharides in F. mume were detected by high performance liquid chromatography, gel permeation chromatography, and gas chromatography-mass spectrometry. The guinea pigs were orally administrated with vehicle or the water extract of Fructus mume (FW) during the 14 days of CS exposure. Citric acid induced coughs were automatically measured by Buxco system. The differential cells in bronchoalveolar lavage fluid (BALF) and histopathological changes in lung tissue were assessed by hematoxylin and eosin staining. The tumor necrosis factor alpha (TNF-alpha) and interleukin-8 (IL-8) levels in lung tissue were detected via enzyme-linked immunosorbent assay. The mucus productions in tracheas were determined with Alcian blue-periodic acid Schiff staining. The results suggested relatively high concentration of citric acid, chlorogenic acid, and neochlorogenic acid in F. mume, and high proportion of galactose and glucose and lower molecular weight of polysaccharides. Administration of FW significantly reduced the cough frequency, decreased inflammatory cells in BALF and lung tissue, and attenuated the thickening of airway epithelium and submucosa compared with CS-exposure group. Moreover, the overproduction of TNF-alpha and IL-8 in lung tissues, and mucus in central airways of CS-induced guinea pigs was markedly inhibited by FW. The extract could also protect against CS exposure-induced chronic cough in guinea pigs by reducing coughs, airways inflammation, and mucus overproduction.