Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice
Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice
复制标题
DOI：
10.1016/j.vaccine.2010.08.096
复制
发表时间：
2010-10-21
期刊：
影响因子：
5.5
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Zhang, Guohong;Huang, Xiaohong;Xuan, Xuenan
刚地弓形虫和犬新孢子虫是密切相关的顶复合体寄生虫。弓形虫的表面抗原1 （TgSAG1）是一种主要的免疫优势抗原，因此被认为是开发有效的重组弓形虫疫苗的良好候选者。本研究以耐乙胺DHFR-TS和GFP基因为双选择标记，构建了稳定表达TgSAG1基因（Nc/TgSAG1）的犬瘟螨。Nc/TgSAG1表达的重组TgSAG1的表达量、分子量和抗原性与天然TgSAG1相似。用Nc/TgSAG1免疫小鼠可诱导TgSAG1特异性th1显性免疫应答，保护小鼠免受弓形虫的致命攻击感染。这些结果表明，该病毒可为动物弓形虫病重组活疫苗的制备提供新的工具。据我们所知，这是第一份评估以犬奈瑟菌为基础的活疫苗有效性的报告。(C) 2010 Elsevier Ltd.版权所有。
Toxoplasma gondii and Neospora caninum are closely related apicomplexan parasites. The surface antigen 1 of T. gondii (TgSAG1) is a major immunodominant antigen and, therefore, is considered to be a good candidate for the development of an effective recombinant vaccine against toxoplasmosis. In this study, N. caninum stably expressing the TgSAG1 gene (Nc/TgSAG1) was constructed using pyrimethamine-resistant DHFR-TS and GFP genes as double-selection markers. The expression level, molecular weight, and antigenic property of recombinant TgSAG1 expressed by the Nc/TgSAG1 were similar to those of the native TgSAG1. The mice immunized with Nc/TgSAG1 induced TgSAG1-specific Th1-dominant immune responses and protected the mice from a lethal challenge infection with T. gondii. These results indicate that N. caninum may provide a new tool for the production of a live recombinant vector vaccine against toxoplasmosis in animals. To our knowledge, this is the first report to evaluate the usefulness of N. caninum-based live vaccine. (C) 2010 Elsevier Ltd. All rights reserved.