Expression of sialyl-Lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues.
Expression of sialyl-Lewis X, an E-selectin ligand, in inflammation, immune processes, and lymphoid tissues.
复制标题
DOI：
--
复制
发表时间：
1992-12
期刊：
影响因子：
--
通讯作者：
J. Munro;S. Lo;C. Corless;M. Robertson;N. Lee;R. Barnhill;D. Weinberg;M. Bevilacqua
中科院分区：
文献类型：
--
作者：
J. Munro;S. Lo;C. Corless;M. Robertson;N. Lee;R. Barnhill;D. Weinberg;M. Bevilacqua
糖结构唾液酸-路易斯X（SLex）可以作为E-选择素的配体起作用，E-选择素以前被称为内皮白细胞粘附分子-1（ELAM-1）。进行本研究以分析在炎症和免疫过程的背景下白细胞和其他细胞类型的SLex的表达。使用针对SLex的单克隆抗体CSLEX 1通过流式细胞术检测人外周血细胞。细胞表面SLex被发现在几乎所有分离的多形核白细胞（PMN）和单核细胞，并在低水平上的一个相当大的部分（高达40%）的自然杀伤细胞。该部分也在约10%的外周血T细胞上表达。对参与炎症或免疫过程的各种人体组织和次级淋巴组织进行免疫组织化学。在急性阑尾炎，毛细血管后微静脉的内皮细胞表达E-选择素，和大多数中性粒细胞，无论是在血管和外渗，表达SLex。大量的单核细胞/巨噬细胞在发炎的aptopheal，滑膜，和皮肤组织也与抗体CSLEX 1反应，然而，只有罕见的组织巨噬细胞在未发炎的nonlymphoid网站显示SLex的表达。这些观察结果与循环PMN和单核细胞上的SLex在炎症反应的发展中作为内皮E-选择素的配体起作用的概念一致。还观察到SLex阳性淋巴细胞，特别是炎症皮肤中的T淋巴细胞。一个意想不到的发现是CSLEX 1抗体也与某些淋巴组织和发炎阑尾中的微静脉内皮反应，但不与正常阑尾中的内皮反应。在内皮上鉴定的SLex抗原是否代表从头表达或被动吸附仍有待确定。
The carbohydrate structure sialyl-Lewis X (SLex) can function as a ligand for E-selectin, formerly known as endothelial leukocyte adhesion molecule-1 (ELAM-1). This study was performed to analyze the expression of SLex by leukocytes and other cell types in the context of inflammatory and immune processes. Human peripheral blood cells were examined by flow cytometry using monoclonal antibody CSLEX1 directed against SLex. Cell surface SLex was found in abundance on nearly all isolated polymorphonuclear leukocytes (PMN) and monocytes, and at low levels on a substantial portion (up to 40%) of natural killer cells. This moiety was expressed also on approximately 10% of peripheral blood T cells. Immunohistochemistry was performed on various human tissues involved in inflammatory or immune processes and on secondary lymphoid tissues. In acute appendicitis, endothelial cells of postcapillary venules expressed E-selectin, and most PMN, both within vessels and extravasated, expressed SLex. A substantial number of monocytes/macrophages in inflamed appendiceal, synovial, and dermal tissues also reacted with antibody CSLEX1; however, only rare tissue macrophages in uninflamed nonlymphoid sites showed expression of SLex. These observations are consistent with the concept that SLex on circulating PMN and monocytes functions as a ligand for endothelial E-selectin in the development of inflammatory reactions. SLex-positive lymphocytes also were seen, notably, T lymphocytes in inflamed skin. An unexpected finding was that the CSLEX1 antibody also reacted with venular endothelium in certain lymphoid tissues and in inflamed appendix, but not with endothelium in normal appendix. Whether the SLex antigen identified on endothelium represents de novo expression or passive adsorption remains to be determined.