Suppression of interleukin 17 production by Brazilian propolis in mice with collagen-induced arthritis
Suppression of interleukin 17 production by Brazilian propolis in mice with collagen-induced arthritis
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DOI：
10.1007/s10787-011-0088-2
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发表时间：
2012-02-01
影响因子：
5.8
通讯作者：
Masuzawa, Toshiyuki
中科院分区：
文献类型：
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作者：
Tanaka, Mayuri;Okamoto, Yoshihiro;Masuzawa, Toshiyuki
蜂胶是蜜蜂从各种植物的叶芽和树皮裂缝中收集的树脂物质。据报道，蜂胶具有免疫调节活性。我们假设蜂胶能够减轻类风湿性关节炎的严重程度。我们评估了巴西蜂胶乙醇提取物对小鼠胶原诱导性关节炎 (CIA) 发病机制的影响。喂食蜂胶的小鼠表现出比喂食对照饮食的小鼠显着更低的临床关节炎评分。为了研究蜂胶对 CIA 小鼠的作用机制，我们使用酶联免疫斑点测定和流式细胞术分析检测了喂食蜂胶的 CIA 小鼠中白细胞介素 17 (IL-17) 的产生。喂食蜂胶的 CIA 小鼠中产生 IL-17 的细胞数量显着减少。为了确定蜂胶对细胞因子产生的直接影响，在蜂胶提取物存在的情况下，用佛波醇肉豆蔻酸酯醋酸酯体外刺激脾细胞。通过 ELISA 和实时 PCR 方法观察到 IL-17 表达浓度依赖性下降。我们进一步发现蜂胶以浓度依赖性方式显着抑制小鼠脾细胞的 Th17 细胞分化。总而言之，我们的结果可能为蜂胶免疫抑制和抗炎作用的潜在机制提供新的线索。
Propolis is a resinous substance collected by honeybees from leaf buds and cracks in the bark of various plants. Propolis has been reported to have immunomodulatory activity. We hypothesized that propolis would be able to reduce the disease severity of rheumatoid arthritis. We evaluated the effect of Brazilian propolis ethanolic extract on the pathogenesis of collagen-induced arthritis (CIA) in mice. Mice fed propolis exhibited significant lower clinical arthritis scores than those fed the control diet. To investigate the mechanism of the effect of propolis on CIA mice, we examined interleukin-17 (IL-17) production in CIA mice fed propolis using an enzyme-linked immunospot assay and flow cytometric analysis. The numbers of IL-17-producing cells in the CIA mice fed propolis were significantly decreased. To determine direct influence of propolis on cytokine production, splenocytes were stimulated with phorbol myristate acetate in the presence of propolis extract in vitro. Concentration-dependent declines in IL-17 expression were observed by ELISA and real-time PCR methods. We further found that propolis significantly inhibited the differentiation of Th17 cells from murine splenocytes in a concentration-dependent manner. Taken together, our results may provide a new light on the potential mechanism of the immunosuppressive and anti-inflammatory effects of propolis.