Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold
Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold
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DOI：
10.1002/anie.200602772
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发表时间：
2006-01-01
影响因子：
16.6
通讯作者：
Marx, Andreas
中科院分区：
文献类型：
--
作者：
Sauter, Katharina B. M.;Marx, Andreas
热稳定DNA聚合酶通常不表现出显著的将RNA逆转录成DNA的内在活性。我们表明，酶的逆转录能力显着增加，可以通过定向进化产生。
Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcribe RNA into DNA. We show that enzymes with significantly increased reverse transcription ability can be generated by directed evolution.