An endosymbiotic bacterium in a plant-parasitic nematode: Member of a new Wolbachia supergroup
An endosymbiotic bacterium in a plant-parasitic nematode: Member of a new Wolbachia supergroup
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DOI：
10.1016/j.ijpara.2009.01.006
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发表时间：
2009-07-15
影响因子：
4
通讯作者：
Gheysen, Godelieve
中科院分区：
文献类型：
--
作者：
Haegeman, Annelies;Vanholme, Bartel;Gheysen, Godelieve
沃尔巴克氏体是一种广泛存在于节肢动物和动物寄生线虫体内的内共生细菌。尽管以前的努力，它从来没有被确定在植物寄生线虫。对穴居线虫相似穿孔线虫（Radopholus similis）表达的基因进行随机测序，得到了几个与沃尔巴克氏体基因相似的序列。利用形态学和分子生物学方法研究了这种植物寄生线虫中存在的Wolbachia样内共生体。透射电子显微镜，荧光免疫定位和DAPI染色证实了内共生体的存在下，女性成年人的生殖道内。16S rDNA、ftsZ和groEL基因序列分析表明，内共生菌的基因序列与内共生菌的基因序列一致。similis与已知的沃尔巴克氏体超类群有远亲关系。最后，基于我们通过筛选表达序列标签（EST）数据集找到这种内共生体序列的初步成功，所有线虫EST都被挖掘为Wolbachia样序列。虽然保留的序列属于六种不同的线虫，但R.相似线虫是唯一带有沃尔巴克氏体痕迹的植物寄生线虫。根据我们的系统发育研究和现有文献，我们将R.与一个新的超群（超群1）相似，而不是将其视为一个新的物种。虽然其作用仍然未知，但在所有测试的个体中发现了内共生体，这表明细菌的基本功能。(C)2009年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Wolbachia is an endosymbiotic bacterium widely present in arthropods and animal-parasitic nematodes. Despite previous efforts, it has never been identified in plant-parasitic nematodes. Random sequencing of genes expressed by the burrowing nematode Radopholus similis resulted in several sequences with similarity to Wolbachia genes. The presence of a Wolbachia-like endosymbiont in this plant-parasitic nematode was investigated using both morphological and molecular approaches. Transmission electron microscopy, fluorescent immunolocalisation and staining with DAPI confirmed the presence of the endosymbiont within the reproductive tract of female adults. 16S rDNA,ftsZ and groEL gene sequences showed that the endosymbiont of R. similis is distantly related to the known Wolbachia supergroups. Finally, based on our initial success in finding sequences of this endosymbiont by screening an expressed sequence tag (EST) dataset, all nematode ESTs were mined for Wolbachia-like sequences. Although the retained sequences belonged to six different nematode species, R. similis was the only plant-parasitic nematode with traces of Wolbachia. Based on our phylogenetic study and the current literature we designate the endosymbiont of R. similis to a new supergroup (supergroup 1) rather than considering it as a new species. Although its role remains unknown, the endosymbiont was found in all individuals tested, pointing towards an essential function of the bacteria. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.