A second chain of human CD8 is expressed on peripheral blood lymphocytes.
A second chain of human CD8 is expressed on peripheral blood lymphocytes.
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人 CD8 的第二条链在外周血淋巴细胞上表达。
DOI：
10.1084/jem.168.6.1993
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Parnes,JR
中科院分区：
文献类型：
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作者：
Shiue,L;Gorman,SD;Parnes,JR
人CD8被认为由与小鼠和大鼠CD8 α同源的单链多肽的二硫键连接的同源二聚体和同源多聚体组成。相反，小鼠和大鼠CD8由α和β链的二硫键连接的异二聚体组成。我们现在已经分离并测序了编码小鼠和大鼠CD8 β的人类同源物的cDNA克隆。将一个这样的克隆插入到表达载体中，并且在与人CD8 α基因共转染到L细胞中后，其编码产物显示在细胞表面上表达。第二种形式的人CD8 β cDNA编码一种具有改变的胞质尾区的蛋白质，也被类似地转染，但其产物不能在细胞表面上显示。CD8 β进一步显示在几乎所有CD8+人外周血T细胞的表面上表达。这些数据提供了人CD8是异二聚体蛋白的第一个证据。
Human CD8 has been thought to consist of disulfide-linked homodimers and homomultimers of a single polypeptide chain homologous to mouse and rat CD8 alpha. In contrast, mouse and rat CD8 are composed of disulfide- linked heterodimers of alpha and beta chains. We have now isolated and sequenced cDNA clones encoding a human homologue of mouse and rat CD8 beta. One such clone was inserted into an expression vector and its encoded product was shown to be expressed on the cell surface after cotransfection into L cells with the human CD8 alpha gene. A second form of human CD8 beta cDNA encoding a protein with an altered cytoplasmic tail was similarly transfected, but its product could not be demonstrated on the cell surface. CD8 beta was further shown to be expressed on the surface of almost all CD8+ human peripheral blood T cells. These data provide the first evidence that human CD8 is a heterodimeric protein.