Interaction of tissue transglutaminase with nuclear transport protein importin-α3
Interaction of tissue transglutaminase with nuclear transport protein importin-α3
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DOI：
10.1016/s0014-5793(99)00018-6
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发表时间：
1999-03-05
期刊：
影响因子：
3.5
通讯作者：
Lokshin, A
中科院分区：
文献类型：
--
作者：
Peng, XJ;Zhang, YH;Lokshin, A
组织转氨酶是一种多功能酶，参与细胞生长、分化和凋亡的调节。最近，tTG的核定位被报道表明具有主动核转运的潜力。用电子显微镜观察了维甲酸（RA）诱导非小细胞肺癌细胞tTG的核表达。这些数据表明，importin-α 3可介导tTG的主动核转运，这可能是重要的细胞过程的调节。(C)1999年欧洲生物化学学会联合会。
Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis, Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport, In this study we use the yeast two-hybrid assay and coimmunoprecipitation to show that tTG interacts with the nuclear transport protein importin-alpha 3. Using electron microscopy we demonstrate that nuclear expression of tTG in a non-small cell lung cancer cell line is induced by retinoic acid (RA), These data suggest that importin-alpha 3 could mediate active nuclear transport of tTG which may be important for the regulation of critical cellular processes. (C) 1999 Federation of European Biochemical Societies.