Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.
Wheat WCBP1 encodes a putative copper-binding protein involved in stripe rust resistance and inhibition of leaf senescence.
复制标题
小麦 WCBP1 编码一种推定的铜结合蛋白，参与抗条锈病和抑制叶片衰老
DOI：
10.1186/s12870-015-0612-4
复制
发表时间：
2015-10-06
影响因子：
5.3
通讯作者：
Luo P
中科院分区：
文献类型：
--
作者：
Li X;Liu T;Chen W;Zhong S;Zhang H;Tang Z;Chang Z;Wang L;Zhang M;Li L;Rao H;Ren Z;Luo P
背景：条锈病是小麦（Triticum aestivum）的一种极具破坏性的叶面病害，它会导致严重的损失，并可能伴随着光合活性降低和叶片衰老加速。方法：采用抑制减法杂交（SSH）技术，对小麦抗性品系L693 （Reg.）的抗性机制进行研究。否。GP-972, PI 672538)，它衍生自一个谱系，包括普通和中介体之间的广泛杂交。对SSH cDNA文库进行测序，鉴定出112个表达的序列标签。结果：通过芯片定位，在1A染色体上找到了其中一个标签[GenBank: JK972238]。基于[GenBank: JK972238]的引物扩增出多态性条带，该条带与YrL693共分离。通过扩增小麦铜结合蛋白（WCBP1）的多态性区，克隆了一个候选基因，并将WCBP1定位在0.64 cM遗传区间。短柄草、水稻和高粱的基因和基因组区域与该区域同源。讨论：序列分析表明，抗性WCBP1等位基因可能是由于缺失了小麦基因组序列的36bp序列，而不是直接从Th转移而来。媒介。qRT-PCR证实WCBP1在病原菌感染后表达发生变化。结论：WCBP1基因独特的染色体定位和表达模式表明，WCBP1基因可能是YrL693的候选基因，参与灌浆期叶片衰老和光合作用，与植物对条锈病的响应有关。
Background:Stripe rust, a highly destructive foliar disease of wheat (Triticum aestivum), causes severe losses, which may be accompanied by reduced photosynthetic activity and accelerated leaf senescence.Methods:We used suppression subtractive hybridization (SSH) to examine the mechanisms of resistance in the resistant wheat line L693 (Reg. No. GP-972, PI 672538), which was derived from a lineage that includes a wide cross between common and Thinopyrum intermedium. Sequencing of an SSH cDNA library identified 112 expressed sequence tags.Results:In silico mapping placed one of these tags [GenBank: JK972238] on chromosome 1A. Primers based on [GenBank: JK972238] amplified a polymorphic band, which co-segregated with YrL693. We cloned a candidate gene encoding wheat copper-binding protein (WCBP1) by amplifying the polymorphic region, and we mapped WCBP1 to a 0.64 cM genetic interval. Brachypodium, rice, and sorghum have genes and genomic regions syntenic to this region.Discussion:Sequence analysis suggested that the resistant WCBP1 allele might have resulted from a deletion of 36-bp sequence of the wheat genomic sequence, rather than direct transfer from Th. intermedium. qRT-PCR confirmed that WCBP1 expression changes in response to pathogen infection.Conclusions:The unique chromosomal location and expression mode of WCBP1 suggested that WCBP1 is the putative candidate gene of YrL693, which was involved in leaf senescence and photosynthesis related to plant responses to stripe rust infection during the grain-filling stage.