Up-Regulation of Crystallins is Involved in the Neuroprotective Effect of Wolfberry on Survival of Retinal Ganglion Cells in Rat Ocular Hypertension Model
Up-Regulation of Crystallins is Involved in the Neuroprotective Effect of Wolfberry on Survival of Retinal Ganglion Cells in Rat Ocular Hypertension Model
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DOI：
10.1002/jcb.22539
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发表时间：
2010-05-15
影响因子：
4
通讯作者：
Chiu, Jin-Fu
中科院分区：
文献类型：
--
作者：
Chiu, Kin;Zhou, Yuan;Chiu, Jin-Fu
枸杞子（枸杞子属）在东方国家有2,500多年的历史，以平衡人体阴阳、滋补肝肾、提高视力而闻名。枸杞中的活性成分主要有枸杞子、枸杞子等。枸杞多糖（LBP）、玉米黄嘌呤、甜菜碱、枸杞苷和微量的锌、铁和铜。每一种都有不同的有益效果，它们共同有助于解释枸杞在东方世界的广泛使用。早期研究报道了枸杞多糖对青光眼实验模型视网膜神经节细胞（retinal ganglion cell，RGC）的神经保护作用，其潜在的体内细胞保护机制值得进一步探讨。本研究采用蛋白质组学、功能基因组学等方法研究枸杞多糖对神经元存活通路的药理作用。在LBP对高眼压视网膜的诱导作用中，仅对晶状体蛋白家族的蛋白质进行了研究。蛋白质组学的结果进一步证实了使用Western印迹的视网膜和免疫组化染色的视网膜切片。我们证明枸杞提取物LBP对RGCs存活的神经保护作用可能通过直接上调神经元存活信号β B2-晶体蛋白介导。J.细胞。110：311-320，2010. (C)2010 Wiley-Liss，Inc.
Wolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of wolfberry extract LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal beta B2-crystallin. J. Cell. Biochem. 110: 311-320, 2010. (C) 2010 Wiley-Liss, Inc.