Enhanced loading of Fura-2/AM calcium indicator dye in adult rodent brain slices via a microfluidic oxygenator.
Enhanced loading of Fura-2/AM calcium indicator dye in adult rodent brain slices via a microfluidic oxygenator.
复制标题
通过微流体充氧器增强成年啮齿动物脑切片中 Fura-2/AM 钙指示剂染料的负载。
DOI：
10.1016/j.jneumeth.2013.04.007
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发表时间：
2013
影响因子：
3
通讯作者：
Eddington,DavidT
中科院分区：
文献类型：
--
作者：
Mauleon,Gerardo;Lo,JoeF;Peterson,BethanyL;Fall,ChristopherP;Eddington,DavidT
微流体氧合器用于向啮齿动物脑切片输送恒定的氧气，使得能够将细胞渗透性钙指示剂Fura-2/AM加载到成人脑切片的细胞中。与传统方法相比，我们的微流控氧合器提高了所有测试年龄组的装载效率，通过每单位面积装载的细胞数量来衡量。实现了1岁小鼠切片的加载，这是目前批量加载方法无法实现的。该技术显著扩大了无需细胞注射即可进行钙研究的年龄范围。这项技术将为研究衰老和长期应激相关疾病的钙信号提供机会。此外，它应该适用于其他膜透性生理指标品种。
A microfluidic oxygenator is used to deliver constant oxygen to rodent brain slices, enabling the loading of the cell-permeant calcium indicator Fura-2/AM into cells of adult brain slices. When compared to traditional methods, our microfluidic oxygenator improves loading efficiency, measured by the number of loaded cells per unit area, for all tested age groups. Loading in slices from 1-year-old mice was achieved, which has not been possible with current bulk loading methods. This technique significantly expands the age range for which calcium studies are possible without cellular injection. This technique will facilitate opportunities for the study of calcium signaling of aging and long term stress related diseases. Moreover, it should be applicable to other membrane-permeant physiological indicator varieties.