Curcumin inhibits collagen synthesis and hepatic stellate cell activation in-vivo and in-vitro
Curcumin inhibits collagen synthesis and hepatic stellate cell activation in-vivo and in-vitro
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DOI：
10.1211/0022357021771823
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发表时间：
2002-01-01
影响因子：
3.3
通讯作者：
Sohn, DH
中科院分区：
文献类型：
--
作者：
Kang, HC;Nan, JX;Sohn, DH
我们以前证明，姜黄素，一个众所周知的抗氧化剂，抑制胶原蛋白沉积在四氯化碳诱导的大鼠肝损伤。在肝脏中负责胶原合成的主要效应细胞是活化的肝星状细胞。在这项研究中，我们研究了姜黄素在体外对大鼠肝星状细胞胶原合成和活化的抑制作用，以及在体内对肝星状细胞活化的抑制作用。在体内和体外研究了姜黄素对胶原蛋白和平滑肌α-肌动蛋白以及α 1（I）胶原蛋白mRNA的产生的影响。姜黄素对DNA合成的影响也在体外测定。在体内，姜黄素治疗减少了胶原沉积和平滑肌α-肌动蛋白阳性区域，并降低了肝脏中I型胶原的mRNA水平。在体外，浓度为5 μ g mL（-1）的姜黄素减少DNA合成，下调平滑肌α-肌动蛋白和I型胶原蛋白表达，以及α 1（I）胶原蛋白mRNA表达。我们的结论是，姜黄素抑制胶原合成和肝星状细胞活化在体内和体外，因此可能被证明是一个有价值的抗纤维化剂。
We previously demonstrated that curcumin, a well-known antioxidant, inhibits collagen deposition in carbon tetrachloride-induced liver injury in rats. The major effector cells responsible for collagen synthesis in the liver are activated hepatic stellate cells. In this study, we investigated the inhibitory effects of curcumin on the collagen synthesis and activation of rat hepatic stellate cells in-vitro, and on hepatic stellate cell activation in-vivo. The effects of curcumin on the production of collagen and smooth muscle alpha-actin proteins and of alpha1(I) collagen mRNA were studied in-vivo and in-vitro. The effect of curcumin on DNA synthesis was also determined in-vitro. In-vivo, treatment with curcumin reduced collagen deposition and smooth muscle a-actin-positive areas and lowered mRNA levels of type I collagen in the liver. In-vitro, curcumin at a concentration of 5 mug mL(-1) reduced DNA synthesis, and downregulated smooth muscle a-actin and type I collagen expression, and alpha1(I) collagen mRNA expression. We concluded that curcumin inhibits collagen synthesis and hepatic stellate cell activation in-vivo and in-vitro, and thus may prove a valuable anti-fibrogenic agent.