The chronic effects of low lead level on the expressions of Nrf2 and Mrp1 of the testes in the rats.
The chronic effects of low lead level on the expressions of Nrf2 and Mrp1 of the testes in the rats.
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DOI：
10.1016/j.etap.2012.12.001
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发表时间：
2013
影响因子：
4.3
通讯作者：
Yan Wang;Jun-cong Fang;Shaoxin Huang;Li Chen;Guangqin Fan;Chunhong Wang
中科院分区：
文献类型：
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作者：
Yan Wang;Jun-cong Fang;Shaoxin Huang;Li Chen;Guangqin Fan;Chunhong Wang
铅与许多生殖问题有关。本研究旨在探讨低铅对大鼠睾丸Nrf2和Mrp1表达的慢性影响。随机分组后，母SD大鼠从妊娠前10天开始至断奶，分别给予3次醋酸铅。每组选取15只雄性幼鼠，分别以0、0.3和0.9g/L的剂量给药，从断奶至6月龄。给药方式为自由饮水。采用RT-PCR、Western blotting和免疫组化方法检测睾丸Mrp1和Nrf2的表达。与对照组相比，两先导组Mrp1、Nrf2的表达均显著升高（P<0.05）；观察Nrf2的细胞核易位；GST和GSH均随铅剂量的增加而降低。综上所述，Mrp1可能在Nrf2对铅的解毒中发挥重要作用。
Lead is linked to many reproductive problems. This study was to explore the chronic effects of low lead level on expressions of Nrf2 and Mrp1 in rats’ testes. Maternal SD rats were administered lead acetate from 10 days before gestation to weaning at three doses respectively after randomization. From each group, 15 male offsprings were then chosen and administrated lead acetate from weaning to six months old at the doses of 0, 0.3 and 0.9g/L respectively. The dose administrations were through drinking water freely. The methods of RT-PCR, Western blotting and immunohistochemistry were used for Mrp1 and Nrf2 of the testes. Compared with control group, significant increases were observed in the expressions of Mrp1 and Nrf2 in two lead groups (P<0.05); nucleus translocation of Nrf2 was observed; both GST and GSH was decreased with increasing the lead dose. In conclusion, Mrp1 might play important roles in lead detoxification by Nrf2.