Diagnostic Efficacy of Saliva For Dengue - A Reality in Near Future? A Piloting Initiative
Diagnostic Efficacy of Saliva For Dengue - A Reality in Near Future? A Piloting Initiative
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DOI：
10.7860/jcdr/2014/7521.4169
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发表时间：
2014-03-01
影响因子：
0.2
通讯作者：
Vidya, G. S.
中科院分区：
文献类型：
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作者：
Banavar, Spoorthi Ravi;Vidya, G. S.
背景：登革热是一种由蚊子传播的病毒感染，会出现多种症状，包括死亡。由于其发病率不断增加，重症病例的早期发现和改进诊断至关重要。目前，病毒抗原和抗体是通过传统的血清学检测来检测的。然而，口腔液作为替代品的引入引发了许多研究。因此，这促使我们开展一项初步研究，以评估使用酶联免疫吸附测定（ELISA）唾液检测登革热抗体的诊断效果。目的和目标：通过ELISA评估唾液中登革热抗体的存在及其敏感性和特异性。方法和结果：分别考虑20名血清阳性登革热患者和20名血清阴性登革热患者。从这些患者收集的唾液样本进行了 ELISA 检测，以检测登革热抗体。诊断登革热感染的敏感性为 100%，特异性为 100%。结论：许多研究利用唾液作为诊断工具，特别是在西方人群中。与静脉穿刺相比，它有很多优点，特别是它侵入性较小、安全、成本较低，并且可以轻松收集大量样本用于筛查和流行病学目的。在像印度这样的热带发展中国家，必须鼓励使用这种诊断工具。进一步的研究需要将唾液作为诊断工具。
Background: Dengue, a mosquito-transmitted viral infection presents variable symptoms, including death. Due to their increasing incidences, early detection and improved diagnoses of severe cases are of prime importance. Currently, viral antigens and antibodies are detected by traditional serological tests. However, the introduction of oral fluid as an alternative, has led to many researches. Hence, this prompted us to carry out a pilot study to evaluate the diagnostic efficacy of saliva in detecting dengue antibody by using Enzyme Linked Immunosorbent Assay (ELISA).Aim and objectives: To evaluate the presence of Dengue antibody in saliva and its sensitivity and specificity through ELISA.Methodology and Results: Twenty seropositive patients and twenty seronegative patients of Dengue were considered individually. Saliva samples collected from these patients were subjected to ELISA test for detection of Dengue antibody. A sensitivity of 100% and a specificity of 100% were obtained for making a diagnosis of Dengue infection.Conclusion: Many studies have been conducted by utilizing saliva as a diagnostic tool, especially in western population. Its advantages over venipuncture are many, especially as it is less invasive, safe, less expensive and as it allows large numbers of samples to be collected easily for screening and epidemiological purposes. In a developing tropical country like India, such a diagnostic tool has to be encouraged. Further research necessitates the implementation of saliva as a diagnostic tool.