EPIDERMAL GROWTH-FACTOR INDUCES PROTEIN-TYROSINE PHOSPHORYLATION AND ASSOCIATION OF P190 WITH RAS-GTP-ASE ACTIVATING PROTEIN IN CACO-2 CELLS
EPIDERMAL GROWTH-FACTOR INDUCES PROTEIN-TYROSINE PHOSPHORYLATION AND ASSOCIATION OF P190 WITH RAS-GTP-ASE ACTIVATING PROTEIN IN CACO-2 CELLS
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DOI：
10.1016/0014-5793(94)00987-2
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发表时间：
1994-10-10
期刊：
影响因子：
3.5
通讯作者：
MIGLIACCIO, A
中科院分区：
文献类型：
--
作者：
AURICCHIO, A;DIDOMENICO, M;MIGLIACCIO, A
表皮生长因子（EGF）调节人肠上皮细胞的多种功能。我们报道，在增殖的人结肠癌细胞（Caco - 2）中，这种生长因子强烈诱导其受体以及受体固有激酶的几种假定底物（包括c - erb B2）的酪氨酸磷酸化刺激。此外，EGF诱导p21（ras）的GTP酶激活蛋白与p190蛋白以及一种62千道尔顿的酪氨酸磷酸化蛋白稳定结合。这种结合可能是EGF刺激蛋白质酪氨酸磷酸化的结果，并且可能协调细胞周期进程与极性、细胞 - 细胞相互作用以及细胞移动性。
Epidermal growth factor (EGF) modulates several functions of human enterocytes. We report that this growth factor induces strong tyrosine phosphorylation stimulation of its receptor and several putative substrates of the receptor intrinsic kinase including c-erb B2 in proliferating human colon carcinoma cells (Caco-2). In addition EGF induces stable association of the GTP-ase activating protein of p21(ras) to the p190 protein and to a 62 mol.wt. tyrosine-phosphorylated protein. This association is probably consequent to EGF stimulation of protein tyrosine phosphorylation and could coordinate progression through cell cycle with polarity, cell-cell interactions and cell mobility.