Uptake of aromatic compounds by DNA: Toward the environmental application of DNA for cleaning water
Uptake of aromatic compounds by DNA: Toward the environmental application of DNA for cleaning water
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DOI：
10.1016/j.colsurfb.2015.03.041
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发表时间：
2015-05-01
影响因子：
5.8
通讯作者：
Murata, Shizuaki
中科院分区：
文献类型：
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作者：
Fernandez-Solis, Christian;Kuroda, Yasuhiro;Murata, Shizuaki
假设：虽然在过去的几十年中，DNA与各种类型的嵌入化学品（如平面多环芳香族化合物）的相互作用已经得到了广泛的研究，但对DNA结合剂的结构与其对DNA的亲和力之间的关系知之甚少。使用DNA作为吸附剂用于环境清洁的目的，需要它的亲和力与不同结构的有机化学品的信息。实验：在本研究中，我们调查了DNA的结合与芳香族化学品的各种结构和电荷通过三种方法：将有机化学品与DNA结合，然后通过用阳离子纳米颗粒（1）或阳离子表面活性剂（2）沉淀除去，结果表明：对于大多数中性有机化合物，有机分子的疏水性是其与DNA有效结合的主要驱动力。DNA的双螺旋性有助于与大多数化合物更强的结合。当疏水阳离子表面活性剂用于DNA复合物的缩合和去除时，有机化学品的吸收效率大幅增加。讨论了DNA作为吸附剂去除水中芳香族有机污染物的潜在环境应用。(C)2015 Elsevier B.V.版权所有。
Hypothesis: Although the interaction of DNA with various types of intercalating chemicals, such as planar polycyclic aromatic compounds, has been extensively investigated over the past several decades, little is known about the relationship between the structure of a DNA binder and its affinity for DNA. The use of DNA as an adsorbent for environmental cleaning purposes requires information on its affinity for organic chemicals with different structures.Experiment: In the present study we investigated the binding of DNA to aromatic chemicals with various structures and charges by three methods: binding of organic chemicals to DNA followed by removal by precipitation with cationic nanoparticles (1) or a cationic surfactant (2), and absorption of organic chemicals by a DNA hydrogel (3).Findings: The results showed that, for most neutral organic chemicals, the hydrophobicity of the organic molecule is the main driving force for efficient binding to DNA. The double-helicity of DNA contributed to stronger binding to most of the compounds. The efficiency of the uptake of organic chemicals increased substantially when a hydrophobic cationic surfactant was used for DNA-complex condensation and removal. The potential environmental application of DNA as an adsorbent for the removal of aromatic organic pollutants from water is discussed. (C) 2015 Elsevier B.V. All rights reserved.