CLOSTRIDIUM-PERFRINGENS AND SOMATIC COLIPHAGES AS INDICATORS OF THE EFFICIENCY OF DRINKING-WATER TREATMENT FOR VIRUSES AND PROTOZOAN CYSTS
CLOSTRIDIUM-PERFRINGENS AND SOMATIC COLIPHAGES AS INDICATORS OF THE EFFICIENCY OF DRINKING-WATER TREATMENT FOR VIRUSES AND PROTOZOAN CYSTS
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DOI：
10.1128/aem.59.8.2418-2424.1993
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发表时间：
1993-08-01
影响因子：
4.4
通讯作者：
FRANCO, E
中科院分区：
文献类型：
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作者：
PAYMENT, P;FRANCO, E
为了找到饮用水中病毒和寄生虫污染的最合适的指标，收集了大量样本，并分析了病原体（可培养的人类肠道病毒，贾第鞭毛虫包囊和隐孢子虫卵囊）和潜在指标（体细胞和男性特异性大肠杆菌，产气荚膜梭菌）的存在。采用常规或更好的处理方法（臭氧氧化、生物过滤）从三个水处理厂采集样品。所有的河水样本都含有所寻找的微生物，只有C。产气荚膜杆菌计数与人肠道病毒、包囊或卵囊相关。对于沉降和过滤的水样，所有指标与人类肠道病毒有统计学相关性，但与包囊或卵囊无相关性。经多元回归分析，体细胞大肠杆菌噬菌体数是澄清水中肠道病毒数的唯一解释变量，而过滤水中则是C。产气荚膜杆菌计数。每个1,000升的成品水样品不含所有微生物，除了含有低水平的包囊和未确定活力的卵囊的单个样品。9个20，000升的成品水样品中有3个显示体细胞大肠杆菌的残留水平为每100升0.03，0.10和0.26。测得的病毒清除率大于4至5 log 10，囊肿清除率大于4 log 10。Coliphage和C.产气荚膜杆菌计数表明总的去除和灭活超过7 log 10活微生物。C.产气荚膜杆菌计数似乎是饮用水处理中灭活和去除病毒的最合适的指示剂。它们似乎也与包囊和卵囊有关，可用作其灭活和去除的指标。我们建议将其用作饮用水病毒和寄生虫检测的替代品。
To find the most suitable indicator of viral and parasitic contamination of drinking water, large-volume samples were collected and analyzed for the presence of pathogens (cultivable human enteric viruses, Giardia lamblia cysts, and Cryptosporidium oocysts) and potential indicators (somatic and male-specific coliphages, Clostridium perfringens). The samples were obtained from three water treatment plants by using conventional or better treatments (ozonation, biological filtration). All samples of river water contained the microorganisms sought, and only C. perfringens counts were correlated with human enteric viruses, cysts, or oocysts. For settled and filtered water samples, all indicators were statistically correlated with human enteric viruses but not with cysts or oocysts. By using multiple regression, the somatic coliphage counts were the only explanatory variable for the human enteric virus counts in settled water, while in filtered water samples it was C. perfringens counts. Finished water samples of 1,000 liters each were free of all microorganisms, except for a single sample that contained low levels of cysts and oocysts of undetermined viability. Three of nine finished water samples of 20,000 liters each revealed residual levels of somatic coliphages at 0.03, 0.10, and 0.26 per 100 liters. Measured virus removal was more than 4 to 5 log10, and cyst removal was more than 4 log10. Coliphage and C. perfringens counts suggested that the total removal and inactivation was more than 7 log10 viable microorganisms. C. perfringens counts appear to be the most suitable indicator for the inactivation and removal of viruses in drinking water treatment. They also appear to have a relationship to cysts and oocysts and could be used as indicators of their inactivation and removal. We propose their use as surrogates for virus and parasite testing of drinking water.