The antioxidant activities and neuroprotective effect of polysaccharides from the starfish Asterias rollestoni.
The antioxidant activities and neuroprotective effect of polysaccharides from the starfish Asterias rollestoni.
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DOI：
10.1016/j.carbpol.2013.02.035
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发表时间：
2013-06
影响因子：
11.2
通讯作者：
Wenjing Zhang;Jing Wang;Weihua Jin;Quanbin Zhang
中科院分区：
文献类型：
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作者：
Wenjing Zhang;Jing Wang;Weihua Jin;Quanbin Zhang
将海星用异丙醇和乙醇脱脂后，用0.15mol/L的盐酸提取粗多糖。采用阴离子交换色谱法将样品分离为SF-1和SF-2两个组分。化学分析表明，SF-1的主要成分是由1→3连接的β-d-葡萄糖醛酸残基为主链的葡聚糖，其次要成分为1→3连接的α-d-葡萄糖醛酸残基为主链的葡聚糖。SF-2是一种甘露葡聚糖硫酸盐。其中SF-2的抗氧化活性最高。此外，SF-1和SF-2在帕金森病（PD）的神经毒性模型中表现出神经保护活性。
After the starfish was defatted with isopropyl alcohol and ethanol, crude polysaccharide was extracted by 0.15mol/L HCl. Anion exchange chromatography was performed to fractionate the sample into two fractions, SF-1 and SF-2. Chemical analysis showed that the major component of SF-1 was a glucan consisting of a backbone of 1→3 linked β-d-glucopyranose residues, and it had a minor glucan component containing a backbone of 1→3 linked α-d-glucopyranose residues. SF-2 was a mannoglucan sulfate. SF-2 displayed the highest antioxidant activity among the polysaccharides. Moreover, SF-1 and SF-2 exhibited neuroprotective activities in a neurotoxicity model of Parkinson's disease (PD).