Trans-endothelial and trans-epithelial transfer of lactoferrin into the brain through BBB and BCSFB in adult rats
Trans-endothelial and trans-epithelial transfer of lactoferrin into the brain through BBB and BCSFB in adult rats
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DOI：
10.1292/jvms.70.313
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发表时间：
2008-03-01
影响因子：
1.2
通讯作者：
Harada, Etsumori
中科院分区：
文献类型：
--
作者：
Kamemori, Nao;Takeuchi, Takashi;Harada, Etsumori
在成年大鼠中通过免疫组织化学研究了静脉注射牛乳铁蛋白（bLF）进入脑脊液（CSF）的情况。输注后10分钟，在脑血管内皮细胞的囊泡膜中检测到所施用的bLF。在脉络丛的室管膜细胞中也检测到许多免疫反应性小囊泡。此外，静脉输注 bLF（10 或 30 mg/kg）后 1-2 小时，脑脊液中 bLF 浓度显着增加。这些发现清楚地表明，即使在成年动物中，LF 也可能被转运到大脑物质中。
The transportation of intravenously administered bovine lactoferrin (bLF) into the cerebrospinal fluid (CSF) was immunohistochemically investigated in adult rats. Administered bLF was detected in the vesicular membranes of endothelial cells in cerebral blood vessels 10 min after the infusion. Numerous immunoreactive small vesicles were also detected at the ependymal cells in the choroid plexus. Moreover, the bLF concentration in the CSF was significantly increased at 1-2 hr after the intravenous infusion of bLF (10 or 30 mg/kg). These findings clearly demonstrate that LF is possibly transported into the brain matter even in adult animals.