Resveratrol protects SR-B1 levels in keratinocytes exposed to cigarette smoke.
Resveratrol protects SR-B1 levels in keratinocytes exposed to cigarette smoke.
复制标题
白藜芦醇可保护暴露于香烟烟雾的角质形成细胞中的 SR-B1 水平。
DOI：
10.1016/j.freeradbiomed.2014.01.007
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发表时间：
2014
影响因子：
7.4
通讯作者：
Valacchi,G
中科院分区：
文献类型：
--
作者：
Sticozzi,C;Belmonte,G;Cervellati,F;Muresan,XM;Pessina,F;Lim,Y;Forman,HJ;Valacchi,G
吸烟（CS）与几种健康状况密切相关，包括心脏病，肺癌和其他呼吸系统和循环系统疾病。吸烟对皮肤的有害影响也有很好的记录，但与对其他器官的影响不同，损害并不取决于吸入。皮肤的上层，即角质层（富含胆固醇脂肪酸和神经酰胺），非常容易受到暴露于环境应激源引起的损伤，环境应激源可以改变其脂质组成，从而影响其保护皮肤免受脱水的功能。清道夫受体B1（SR-B1）参与包括皮肤在内的多种组织中胆固醇的摄取。我们以前证明，CS暴露诱导醛（HNE）加合物的形成，降低SR-B1的表达。作为局部白藜芦醇，一个众所周知的多酚芪，已被证明对皮肤疾病的好处，我们研究了其可能的作用，作为一种保护剂，对CS诱导的SR-B1在皮肤组织中的表达减少。在这项研究中，我们证明了白藜芦醇在0.5至10 μM的剂量范围内是无毒的，并且能够以剂量依赖的方式增加人角质形成细胞中SR-B1蛋白水平。此外，当用不同剂量的白藜芦醇预处理的细胞暴露于CS时，SR-B1的损失以剂量依赖性的方式被阻止。此外，在角质形成细胞中，白藜芦醇也能够防止CS诱导的HNE-蛋白加合物的增加。特别是白藜芦醇能够防止HNE-SR-B1加合物的形成。因此，白藜芦醇似乎是一种天然化合物，可以通过保护必需的胆固醇受体SR-B1来为皮肤提供抵御外源性压力的防御。
Cigarette smoking (CS) has been strongly linked to several health conditions including heart disease, lung cancer, and other respiratory and circulatory ailments. Deleterious effects of cigarette smoking on skin have also been well documented, but unlike effects on other organs, damage does not depend upon inhalation. The upper layer of the skin, the stratum corneum (rich in cholesterol fatty acids and ceramide), is very susceptible to damage induced by exposure to environmental stressors that can modify its lipid composition and thereby affect its function of protecting skin from dehydration. Scavenger receptor B1 (SR-B1) is involved in the uptake of cholesterol in several tissues including skin. We previously demonstrated that CS exposure induces formation of aldehyde (HNE) adducts that decrease SR-B1 expression. As topical resveratrol, a well-known polyphenolic stilbene, has been demonstrated to show benefits against skin disorders, we investigated its possible role as a protective agent against CS-induced reduction of SR-B1 expression in cutaneous tissue. In this study, we demonstrate that resveratrol at doses ranging from 0.5 to 10 μM is not toxic and is able to increase SR-B1 protein levels in a dose-dependent manner in human keratinocytes. Moreover, when the cells that were pretreated with various doses of resveratrol were exposed to CS, the loss of SR-B1 was prevented in a dose-dependent manner. In addition, in keratinocytes, resveratrol was also able to prevent an increase in HNE–protein adducts induced by CS. In particular resveratrol was able to prevent HNE–SR-B1 adduct formation. Thus, resveratrol seems to be a natural compound that could provide skin with a defense against exogenous stressors by protecting the essential cholesterol receptor, SR-B1.