Vascular protein leakage in the rat parotid gland elicited by reflex stimulation, parasympathetic nerve stimulation and administration of neuropeptides
Vascular protein leakage in the rat parotid gland elicited by reflex stimulation, parasympathetic nerve stimulation and administration of neuropeptides
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DOI：
10.1016/s0167-0115(98)00121-9
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发表时间：
1998-10-16
影响因子：
--
通讯作者：
Ekström, J
中科院分区：
文献类型：
--
作者：
Asztély, A;Havel, G;Ekström, J
清醒大鼠在没有阿托品和肾上腺素受体拮抗剂的情况下，以及在感觉神经毒素辣椒素预处理后，清醒大鼠腮腺中的伊文思蓝在摄食后(超过60min)积累。刺激耳颞神经(40赫兹，10或20分钟)，在没有或有阻滞剂的情况下，导致伊文思蓝积聚。腺体周围的水肿区也含有染料。管理(静脉注射)而P物质、血管活性肠肽(VIP)、垂体腺苷环化酶激活肽(PACAP)、降钙素基因相关肽(CGRP)和匹罗卡品则无此作用。匹罗卡品可增强神经激动素A的作用，P物质与VIP、PACAP或CGRP共同作用可引起伊文思蓝蓄积。在舌下+颌下腺，神经激动素A和匹罗卡品使伊文思蓝增加，P物质和VIP、P物质和CGRP正相互作用。缓激肽在腺体中缺乏作用。并与膀胱进行比较。伊文思蓝的积聚反映了血浆蛋白的外渗。在唾液腺中，这种现象发生在进食过程中，与完整的感觉神经支配无关；相反，含有神经肽的副交感神经支配是焦点。在临床上，目前的发现可能对腺体肿胀和疼痛的病因有一定的影响。(C)1998 Elsevier Science B.V.保留所有权利。
Evans blue accumulated in parotid glands of conscious rats in response to feeding (over 60 min), in the absence of atropine and adrenoceptor antagonists and in their presence, and after pretreatment with the sensory neurotoxin capsaicin. Stimulation of the auriculo-temporal nerve (40 Hz, 10 or 20 min), without and with the blockers, caused Evans blue to accumulate. A periglandular oedema also contained the dye. Administration (i.v.) of neurokinin A accumulated Evans blue, while substance P, vasoactive intestinal peptide (VIP), pituitary adenylate cyclase-activating peptide (PACAP), calcitonin gene-related peptide (CGRP) and pilocarpine lacked effect. Pilocarpine enhanced the action of neurokinin A and, furthermore, substance P combined with either VIP, PACAP or CGRP resulted in accumulation of Evans blue. In the sublingual + submandibular glands, Evans blue increased in response to neurokinin A and pilocarpine; furthermore, substance P and VIP, and substance P and CGRP, interacted positively. Bradykinin lacked effect in the glands. Comparisons were made with the urinary bladder. Accumulation of Evans blue reflects plasma protein extravasation. In salivary glands, the phenomenon occurred during feeding and was independent on intact sensory innervation; instead, the parasympathetic innervation containing the neuropeptides was in focus. In the clinic, the present findings may have implications for the aetiology of gland swelling and pain. (C) 1998 Elsevier Science B.V. All rights reserved.