Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex
Luminescence-based colorimetric discrimination of single-nucleotide transversions by the combined use of the derivatives of DOTA-conjugated naphthyridine and its terbium complex
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DOI：
10.1016/j.tetlet.2009.02.152
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发表时间：
2009-05-13
影响因子：
1.8
通讯作者：
Nagasaki, Yukio
中科院分区：
文献类型：
--
作者：
Atsumi, Hiroshi;Yoshimoto, Keitaro;Nagasaki, Yukio
我们新合成了一个核碱基结合配体ND-DOTA，其中2-氨基-5，7-二甲基-1，8-萘啶（ND）通过酰胺连接基与1，4，7，10-四氮杂环十二烷-1，4，7-三乙酸（DOTA）偶联，并发现其铽（III）配合物（ND-DOTA-Tb）显示基于萘啶部分到Tb 3+的能量转移的绿色发射。通过添加含有脱碱基位点的DNA双链体（其具有与脱碱基位点相对的嘧啶碱基）来选择性地淬灭ND-DOTA的蓝色发射。与此相反，在相同的激发波长下，ND-DOTA-Tb显示绿色发射，而与无碱基位点相对的碱基无关。因此，ND-DOTA和ND-DOTA-Tb的混合溶液使得能够在单个激发波长下用肉眼对单核苷酸颠换进行基于发光的比色辨别。(C)2009爱思唯尔有限公司保留所有权利。
We newly synthesized a nucleobase-binding ligand, ND-DOTA, in which 2-amino-5,7-dimethyl-1,8-naphthyridine (ND) was conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DOTA) via an amide linker, and found that its terbium(III) complex (ND-DOTA-Tb) showed green emission based on an energy transfer from the naphthyridine moiety to Tb3+. The blue emission of ND-DOTA was selectively quenched by adding abasic site-containing DNA duplexes that have pyrimidine bases opposite to the abasic site. In contrast, at the same excitation wavelength, ND-DOTA-Tb showed green emission independently of the bases opposite to the abasic site. Thus, a mixed solution of ND-DOTA and ND-DOTA-Tb enabled the luminescence-based colorimetric discrimination of single-nucleotide transversions with the naked eye at a single excitation wavelength. (C) 2009 Elsevier Ltd. All rights reserved.