A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection.
A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection.
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DOI：
10.1039/d0cc02072a
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发表时间：
2020-05
影响因子：
4.9
通讯作者：
Shengyu Chen;Jingjin Zhao;Xing Yang;Shulin Zhao;Yi-Ming Liu
中科院分区：
文献类型：
--
作者：
Shengyu Chen;Jingjin Zhao;Xing Yang;Shulin Zhao;Yi-Ming Liu
建立了一种细胞内信号放大技术，用于单细胞内ATP的微芯片电泳-激光诱导荧光检测。用该方法测定了单个HeLa细胞、HepG 2细胞和HL-7702细胞内ATP含量，其范围分别为30-150、30-140和19-120 fmol/细胞。
An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells were found to be in the range of 30-150, 30-140, and 19-120 fmol per cell, respectively.