LOWRY METHOD OF PROTEIN QUANTIFICATION - EVIDENCE FOR PHOTOSENSITIVITY

LOWRY METHOD OF PROTEIN QUANTIFICATION - EVIDENCE FOR PHOTOSENSITIVITY
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DOI:
10.1016/0003-2697(84)90183-0
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发表时间:
1984-01-01
影响因子:
2.9
通讯作者:
HEATLIE, PL
HEATLIE, PL
中科院分区:
生物学4区
文献类型:
--
作者:
DAWSON, JM;HEATLIE, PL

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尽管有报告称其易受各种干扰因素的影响,但Folin Phenol蛋白质定量方法等(1951)仍然是常规蛋白质测定的最方便和准确的方法。Lowry检测试剂盒也具有光敏性,除非采取适当的预防措施,否则可能会导致估计的蛋白质浓度出现高达10%的差异。
Despite reports of its susceptibility to various interfering factors, the Folin Phenol protein quantification method et al. (1951) remains the most convenient and accurate method for routine protein determinations. The Lowry assay is also photosensitive, which can result in a discrepancy of up to 10% in estimated protein concentrations unless appropriate precautions are taken.