The red flour beetle's large nose:: An expanded odorant receptor gene family in Tribolium castaneum
The red flour beetle's large nose:: An expanded odorant receptor gene family in Tribolium castaneum
复制标题
DOI：
10.1016/j.ibmb.2007.10.005
复制
发表时间：
2008-04-01
影响因子：
3.8
通讯作者：
Brown, Stephen
中科院分区：
文献类型：
--
作者：
Engsontia, Patamarerk;Sanderson, Alan P.;Brown, Stephen
赤拟谷盗基因组序列揭示了大量的气味受体（或）基因相比，在其他昆虫的嗅觉基因组已被研究-341或基因和假基因，编码259个完整的气味受体蛋白。幼虫和成虫的RT-PCR研究表明，只有145（64%）的233个基因成功的基因组DNA扩增的表达。在任何年龄都没有检测到其他87个基因的表达，这表明这些基因在这个特定的菌株中不表达，或者它们只在某些环境或发育条件下被诱导。TcOR 1是果蝇Or 83 b（DmOr 83 b）基因的直系同源物，该基因是果蝇嗅觉受体蛋白功能所必需的，在成年和幼虫头部提取物和成年体提取物中表达。在来自幼虫头部组织的提取物中检测到41个TcOr基因的表达，并且在来自成虫头部组织的提取物中检测到111个TcOr基因的表达（两个图均排除TcOr 1）。28个TcOrs仅在成人体内检测到。给甲虫蛹注射TcOr 1 dsRNA;与假注射和对照甲虫不同，这些敲除甲虫对拟谷盗聚集信息素没有显示出显著的反应，支持TcOr 1在嗅觉中起与DmOr 83 b类似的决定性作用的假设。大量的Ors提出了一个问题，为什么赤拟谷盗有这么大的嗅觉受体库，并强调需要更多的研究这个物种的自然历史。(C)2007爱思唯尔有限公司版权所有。
The Tribolium castaneum genome sequence reveals a large number of odorant receptor (Or) genes compared to those found in other insects whose olfactory genomes have been studied-341 Or genes and pseudogenes, encoding 259 intact odorant receptor proteins. An RT-PCR study of larvae and adults revealed that only 145 (64%) of 233 genes with successful genomic DNA amplifications were expressed. No expression of the other 87 genes was detected at any age, suggesting either that these genes are not expressed in this particular strain, or that they are induced only in certain environmental or developmental conditions. TcOR1, the ortholog of the Drosophila Or83b (DmOr83b) gene, which is required for the function of olfactory receptor proteins in Drosophila, was expressed in extracts from adult and larval heads and in extracts from adult bodies. Expression of 41 TcOr genes was detected in extracts from larval head tissue and 1 1 1 in extracts from adult head tissue (both figures exclude TcOr1). Twenty-eight TcOrs were detected only in adult bodies. Beetle pupae were injected with TcOr1 dsRNA; unlike sham-injected and control beetles, these knock-down beetles showed no significant response to the Tribolium aggregation pheromone, supporting the hypothesis that TcOr1 plays a similar decisive role in olfaction to DmOr83b. The substantial number of Ors poses the question of why Tribolium has such a large olfactory receptor repertoire, and underlines the need for more studies of the natural history of this species. (C) 2007 Elsevier Ltd. All rights reserved.