Myosin heavy chain 10 (MYH10) is required for centriole migration during the biogenesis of primary cilia
Myosin heavy chain 10 (MYH10) is required for centriole migration during the biogenesis of primary cilia
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DOI：
10.1016/j.bbrc.2015.04.028
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发表时间：
2015-05-22
影响因子：
3.1
通讯作者：
Kim, Joon
中科院分区：
文献类型：
--
作者：
Hong, Hyowon;Kim, Jongshin;Kim, Joon
肌动蛋白细胞骨架与纤毛的组装有关，但肌动蛋白依赖的运动蛋白在纤毛发生中的作用尚不清楚。肌球蛋白重链10（MYH 10），非肌肉肌球蛋白II的亚型之一，已知介导细胞迁移过程中的中心体重定向。在这里，我们表明，MYH 10是所需的中心粒迁移到顶端质膜，发生在纤毛发生。RPE 1细胞中MYH 10的敲低导致皮质丝状肌动蛋白（F-肌动蛋白）及其结合蛋白EZRIN水平的降低。此外，无论是中心粒迁移和随后的纤毛组装缺陷MYH 10耗尽细胞。我们进一步发现，MYH 10影响了中心体对IFT 88的募集，这是将结构单元运输到纤毛尖端所必需的。MYH 10在IFT 88募集中的作用似乎是间接的，因为在纤毛发生过程中，中心粒IFT 88水平和中心粒位置沿着顶基轴之间存在相关性。我们的研究结果表明，MYH 10有助于通过促进皮层肌动蛋白依赖的中心粒迁移在RPE 1细胞的纤毛发生。(C)2015 Elsevier Inc. All rights reserved.
The actin cytoskeleton has been implicated in the assembly of cilia, but roles of actin-dependent motor proteins in ciliogenesis remain unclear. Myosin heavy chain 10 (MYH10), one of the isoforms of non-muscle myosin II, is known to mediate centrosome reorientation during cell migration. Here we show that MYH10 is required for centriole migration to the apical plasma membrane, which occurs at the onset of ciliogenesis. Knockdown of MYH10 in RPE1 cells caused a reduction in the levels of cortical filamentous actin (F-actin) and its binding protein EZRIN. Moreover, both centriole migration and subsequent cilium assembly were defective in MYH10 depleted cells. We further found that MYH10 influences centrosomal recruitment of IFT88, which is required for the transport of building blocks to the ciliary tip. The role of MYH10 in IFT88 recruitment appears to be indirect in that there is a correlation between centriolar IFT88 levels and centriolar positions along the apical-basal axis during ciliogenesis. Our results indicate that MYH10 contributes to ciliogenesis in RPE1 cells by promoting cortical actin-dependent centriole migration. (C) 2015 Elsevier Inc. All rights reserved.