Antiviral effect of vitamin A on norovirus infection via modulation of the gut microbiome.
Antiviral effect of vitamin A on norovirus infection via modulation of the gut microbiome.
复制标题
DOI：
10.1038/srep25835
复制
发表时间：
2016-05-16
影响因子：
4.6
通讯作者：
Ko G
中科院分区：
文献类型：
--
作者：
Lee H;Ko G
维生素A对诺如病毒感染的作用和潜在机制在很大程度上是未知的。本研究旨在探讨维生素A对诺如病毒感染后肠道微生物群的影响。在这里，我们证明用视黄醇或视黄酸（RA）治疗可以抑制小鼠诺如病毒（MNV）在体外和体内模型中的复制。还调查了与类风湿关节炎给药和/或诺如病毒感染相关的肠道微生物组的组成变化。口服RA和/或MNV可显著改变肠道微生物群。特别是，接种MNV和给予RA后，乳酸杆菌科的细菌种类显著增加，这表明RA的抗病毒作用是通过调节特定的微生物群来实现的。在RAW264.7细胞的体外模型中鉴定并证实了乳杆菌的抗病毒因果作用。对MNV的抗病毒免疫应答是由IFN-β上调介导的。这项研究首次全面分析了肠道微生物群对类风湿关节炎治疗对抗诺如病毒感染的反应。此外，我们得出结论，通过RA调节肠道微生物群的乳酸杆菌丰度至少部分负责诺如病毒抑制。
The effect and underlying mechanism of vitamin A on norovirus infection are largely unknown. This study aimed to investigate how vitamin A administration affects the gut microbiome after norovirus infection. Here, we demonstrate that treatment with either retinol or retinoic acid (RA) inhibits murine norovirus (MNV) replication using both in vitro and in vivo models. Compositional changes in the gut microbiome associated with RA administration and/or norovirus infection were also investigated. Oral administration of RA and/or MNV significantly altered intestinal microbiome profiles. Particularly, bacterial species belonging to the Lactobacillaceae families were remarkably increased by MNV inoculation and RA administration, suggesting that the antiviral effects of RA occur via the modulation of specific microbiota. The antiviral causal effect of Lactobacillus was identified and demonstrated using in vitro models in RAW264.7 cells. The antiviral immune response to MNV was mediated by IFN-β upregulation. This study represents the first comprehensive profiling of gut microbiota in response to RA treatment against norovirus infection. Moreover, we conclude that the abundance of Lactobacillus through gut microbiota modulation by RA is at least partially responsible for norovirus inhibition.