Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain
Crosslinking density influences the morphology of chondrocytes photoencapsulated in PEG hydrogels during the application of compressive strain
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DOI：
10.1016/j.orthres.2004.02.001
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发表时间：
2004-09-01
影响因子：
2.8
通讯作者：
Bader, DL
中科院分区：
文献类型：
--
作者：
Bryant, SJ;Anseth, KS;Bader, DL
机械负荷期间发生的软骨细胞变形被认为在机械传导途径中发挥重要作用。在设计用于软骨组织工程的可原位凝胶化的支架时，重要的考虑因素是机械载荷的影响。本研究测试了水凝胶支架的交联密度的变化影响封装的软骨细胞响应于施加的负载的形态的假设。软骨细胞被截留在基于聚（乙二醇）（PEG）的光交联水凝胶支架中，具有两种交联密度，0.119和0.376 mol/l，较高的密度具有11倍的压缩模量。在培养1至6天后，使细胞包埋的水凝胶经受0%至20%的静态压缩应变。使用共聚焦激光扫描显微镜，软骨细胞在高度交联的凝胶在第1天变形比凝胶在更松散的交联凝胶。到第6天，这一发现被逆转。当一个区域内的单细胞进行了跟踪，在宏观和微观尺度上观察到细胞变形的不均匀性。这些异质性在高度交联的凝胶中更大。这些发现表明，通过改变PEG水凝胶中的交联密度可以获得不同水平的细胞变形和异质性。(C)2004骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
Chondrocyte deformation, which occurs during mechanical loading, is thought to play an important role in the mechanotransduction pathway. In designing a scaffold that can be gelled in situ for cartilage tissue engineering, an important consideration is the influence of mechanical loading. This study tested the hypothesis that changes in the crosslinking density of a hydrogel scaffold influence the morphology of encapsulated chondrocytes in response to an applied load. Chondrocytes were entrapped in photocrosslinkable hydrogel scaffolds based on poly(ethylene glycol) (PEG) with two crosslinking densities, 0.119 and 0.376 mol/l, with the higher density having a 11-fold higher compressive modulus. The cell-embedded hydrogels were subjected to static compressive strains between 0% and 20% after 1 and 6 days of culture. Using confocal laser scanning microscopy, chondrocytes in the highly crosslinked gel at day 1 deformed more than gels in the more loosely crosslinked gel. By day 6, this finding was reversed. When single cells within a region were followed, heterogeneities in cell deformation were observed on both a macroscopic and microscopic scale. These heterogeneities were greater in the highly crosslinked gel. These findings demonstrate that different levels of cell deformation and heterogeneity may be obtained by varying the crosslinking density in PEG hydrogels. (C) 2004 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.