TBCD Links Centriologenesis, Spindle Microtubule Dynamics, and Midbody Abscission in Human Cells
TBCD Links Centriologenesis, Spindle Microtubule Dynamics, and Midbody Abscission in Human Cells
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DOI：
10.1371/journal.pone.0008846
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发表时间：
2010-01-22
期刊：
影响因子：
3.7
通讯作者：
Carlos Zabala, Juan
中科院分区：
文献类型：
--
作者：
Lopez Fanarraga, Monica;Bellido, Javier;Carlos Zabala, Juan
Microtubule-organizing centers recruit alpha- and beta-tubulin polypeptides for microtubule nucleation. Tubulin synthesis is complex, requiring five specific cofactors, designated tubulin cofactors (TBCs) A-E, which contribute to various aspects of microtubule dynamics in vivo. Here, we show that tubulin cofactor D (TBCD) is concentrated at the centrosome and midbody, where it participates in centriologenesis, spindle organization, and cell abscission. TBCD exhibits a cell-cycle-specific pattern, localizing on the daughter centriole at G1 and on procentrioles by S, and disappearing from older centrioles at telophase as the protein is recruited to the midbody. Our data show that TBCD overexpression results in microtubule release from the centrosome and G1 arrest, whereas its depletion produces mitotic aberrations and incomplete microtubule retraction at the midbody during cytokinesis. TBCD is recruited to the centriole replication site at the onset of the centrosome duplication cycle. A role in centriologenesis is further supported in differentiating ciliated cells, where TBCD is organized into "centriolar rosettes". These data suggest that TBCD participates in both canonical and de novo centriolar assembly pathways.
