Cn3D: a new generation of three-dimensional molecular structure viewer
Cn3D: a new generation of three-dimensional molecular structure viewer
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DOI：
10.1016/s0968-0004(97)01093-1
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发表时间：
1997-08-01
影响因子：
13.8
通讯作者：
Hogue, CWV
中科院分区：
文献类型：
--
作者：
Hogue, CWV
具有ECL套件组件的自制解决方案的结果表明，它们的工作效果与商业解决方案一样好或更好。网民们吹嘘说，许多带有ECL套件组件的自制解决方案表明，它们的效果与商业解决方案一样好，甚至更好。网友们吹嘘说，新自制的鲁米诺甚至可以比从公司购买的卢米诺的灵敏度高出一倍。值得一提的是，ECL增强剂的使用是受专利保护的。为了节省更多的钱在西部，一些网民说，他们重复使用他们的主要抗体多达15次，而不会失去信号。当使用生物素标记的探针时，Netters报告说，通过将膜预先孵育在1%的精制酪蛋白溶液中作为封闭剂，而不是使用BLQTTO或脱脂奶粉，可以获得较低的本底，后者有时可能含有不需要的生物素。脱脂干奶中的酪蛋白可用pH 4.6的沉淀酸和0.1~NaOH溶液进行部分纯化。8)。去除锡的提纯可以通过链霉亲和素-琼脂糖柱完成。LA后大片段DNA的麻烦--可能是转化效率很低。作为回应，在一项关于大质粒20kb(参考文献)的转化效率的研究中，使用了4个单位的DNA gic~‘orics luteus(Topoi)在tra之前将DNA缠绕在一起。91，转化效率比未处理的DNA提高了九倍。
of homemade solutions with ECL kit com-ponents show they work as well or better than the commercial ones. Netters brag that fre of homemade solutions with ECL kit components show they work as well or better than the commercial ones. Netters brag that freshly made homemade luminol can even give up to twofold more sensitivity than that bought from companies. It should be mentioned, though, that the use of enhancers for ECL is covered by patent. To save even more money on westerns, some netters say they re-use their primary antibodies up to 15 times without loss of signal. When using biotin-labeled probes, netters report that lower background can be obtained by pre-incubating the membrane in a solution of 1% purified casein as blocking agent, rather than using BLQTTO or non-fat dry milk, which can sometimes contain unwanted biotin. Casein can be partially purified from nonfat dry milk by precipf acetic acid, pH 4.6, and th it in 0.1~ NaOH (Ref. 8). purification to remove a tin can be done by pass through a streptavidin-agarose column. troubles ng large fragments of DNA after LA- It might be that the transformation efficiency is little success. in response, one using 4 units of DNA gy c~‘ococcus luteus (topoi wind up the DNA before tra In a study on the transfo ciency of large plasmids 20 kb (Ref. 91, a ninefold increase i transformation efficiency was obtaine as compared to DNA not treated with