Comprehensive proteomic analysis of the human amniotic fluid proteome: Gestational age-dependent changes
Comprehensive proteomic analysis of the human amniotic fluid proteome: Gestational age-dependent changes
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DOI：
10.1021/pr060543t
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Nagalla, Srinivasa R.
中科院分区：
文献类型：
--
作者：
Michaels, John-Edward A.;Dasari, Surendra;Nagalla, Srinivasa R.
羊水是胎儿健康的重要因素，是诊断母婴疾病的重要生物标志物。在这项研究中，我们对在AF中表达的蛋白质进行了全面的调查，结合了凝胶和液体分离方法以及LC-MS/MS分析。二维液相色谱(2D-LC)分析高置信度地鉴定了118个非冗余蛋白质。一、二维凝胶电泳法和凝胶内切酶鉴定了101种蛋白质。将这两组结合在一起产生了219种蛋白质，其中96种是房颤特有的；血清、宫颈阴道液和所有三种液体中分别存在70、18和35种蛋白质。荧光二维差示凝胶内电泳法(2D-DGE)对妊娠早期、中期和晚期的羊水样本进行比较，发现房颤蛋白相对丰度的最大差异出现在早孕和中期之间。对房颤和母体血清中存在的蛋白质进行系统分析，可能会导致开发新的非侵入性诊断程序来监测胎儿状况。
Amniotic fluid (AF) is a significant contributor to fetal health and constitutes a potential rich source of biomarkers for diagnosis of maternal and fetal disorders. In this study, we performed a comprehensive survey of the proteins expressed in AF, combining gel and liquid-based fractionation approaches coupled with LC-MS/MS analysis. Two-dimensional Liquid Chromatography (2D-LC) analysis identified 118 nonredundant proteins with high confidence. One- and two-dimensional gel electrophoresis and in-gel digestion identified 101 proteins. Combining both sets resulted in 219 proteins, of which 96 are unique to AF; 70, 18, and 35 proteins are present in serum, cervico-vaginal fluid, and all three fluids, respectively. Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) comparison of first-, second-, and third-trimester AF samples revealed that maximal differences in the relative abundance of AF proteins occur between the first and second trimesters. A systematic analysis of proteins present both in AF and maternal serum could lead to the development of new noninvasive diagnostic procedures to monitor fetal status.