Regulation of IL-2 beta receptor expression and beta-chain mRNA by human thymocytes.
Regulation of IL-2 beta receptor expression and beta-chain mRNA by human thymocytes.
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人类胸腺细胞对 IL-2 β 受体表达和 β 链 mRNA 的调节。
DOI：
10.1111/j.1365-2249.1992.tb06429.x
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发表时间：
1992
影响因子：
4.6
通讯作者：
Marcelli,A
中科院分区：
文献类型：
--
作者：
Reem,GH;Han,X;Marcelli,A
人IL-2受体（IL-2R）的高亲和力形式具有两种已知组分，IL-2R α（p55）和IL-2R β链（p75）。我们先前已经证明，重组IL-2（rIL-2）可以诱导T细胞和胸腺细胞上α链（p55）的表达，并在伴刀豆球蛋白A（Con A）与IL-2联合激活后增加这种表达。还记录了rIL-2诱导T细胞和胸腺细胞中IL-2R α特异性mRNA积累的增加。我们在此报告，IL-2R β在细胞表面的表达可以通过Mik β 1（一种针对β链表位的单克隆抗体）的结合在人胸腺细胞上得到证实。IL-2R β链在新鲜分离的胸腺细胞上组成性表达;在用Con A与IL-2或十四烷酰佛波醇13-乙酸酯（TPA）组合活化的胸腺细胞中，该表达可以增加。用针对受体α链的MoAb阻断高亲和力受体的形成导致IL-2R β的表达增加，如MoAb Mik β 1结合所证明。在新鲜分离的胸腺细胞中观察到IL-2R β特异性mRNA的积累，并且在单独用rIL-2和Con A培养的胸腺细胞中增加。并通过加入rIL-2与Con A或TPA的组合而进一步增强。环孢菌素（CsA）可抑制胸腺细胞淋巴因子特异性mRNA的积聚，但不抑制IL-2 R β特异性mRNA积聚的诱导。这与其对α链（p55）表达和α链特异性mRNA积累的作用类似。
The high affinity form of the human IL-2 receptor (IL-2R) has two known components, the IL-2Rα (p55) and the IL-2Rβ chain (p75). We have previously shown that recombinant IL-2 (rIL-2) could induce the expression of the α-chain (p55) on T cells and thymocytes, and increase this expression following suboptimal activation with concanavalin A (Con A) in combination with IL-2. An increase in the accumulation of IL-2Rα-specific mRNA induced by rIL-2 in T cells and thymocytes had also been documented. We report here that the expression of IL-2Rβ on the cell surface can be demonstrated on human thymocytes by the binding of Mik β1, a MoAb directed against an epitope of the β-chain. The IL-2Rβ chain is constitutively expressed on freshly isolated thymocytes; this expression can be increased in thymocytes activated with Con A in combination with IL-2 or tetradecanoylphorbol 13-acetaie (TPA). Blocking the formation of high affinity receptors with a MoAb directed against the α-chain of the receptor results in an increase in the display of IL-2Rβ as evidenced by binding of MoAb Mik β1. The accumulation of IL-2Rβ specific mRNA is observed in freshly isolated thymocytes and it is increased in thymocytes cultured with rIL-2 alone, with Con A. and further enhanced by the addition of rIL-2 in combination with Con A or with TPA. Cyclosporine (CsA), which inhibits the accumulation of lymphokine-specific mRNA of thymocytes, does not inhibit the induction of the accumulation orIL-2Rβ-specific mRNA. This is analogous to its effect on the expression of the α-chain (p55), and the accumulation of α-chain-specific mRNA.