Comparison of antitumor activities of different polysaccharide fractions from the stems of Dendrobium nobile Lindl
Comparison of antitumor activities of different polysaccharide fractions from the stems of Dendrobium nobile Lindl
复制标题
金钗石斛茎不同多糖组分的抗肿瘤活性比较
DOI：
10.1016/j.carbpol.2009.07.032
复制
发表时间：
2010-01-05
影响因子：
11.2
通讯作者：
Feng, Bao-Jun
中科院分区：
文献类型：
--
作者：
Wang, Jun-Hui;Luo, Jian-Ping;Feng, Bao-Jun
研究了金钗石斛茎多糖的抗肿瘤活性。从石斛茎中提取多糖。从金鱼草中分离得到水提取物（DNP-W）、5%NaOH提取物（DNP-OH）和5%HCl提取物（DNP-H）。进一步通过阴离子交换色谱法分离DNP-W，得到6个亚级分（DNP-W1、DNP-W2、DNP-W3、DNP-W 4、DNP-W5和DNP-W 6）。分析了9个多糖组分的单糖组成、蛋白质含量、糖醛酸含量、总糖含量、粘度和分子量。对9个多糖组分进行了体内外抗肿瘤活性评价和比较。结果表明，DNP-W1和DNP-W3在体内对肉瘤180和体外对HL-60细胞具有较强的抗肿瘤活性。结果表明，DNP-W1和DNP-W3是一种有效的天然抗肿瘤源。(C)2009爱思唯尔有限公司保留所有权利。
The antitumor activities of extracted polysaccharide fractions from the stems of Dendrobium nobile Lindl were investigated. Polysaccharides were sequentially extracted from the stems of D. nobile to obtain three fractions, i.e. water extract fraction (DNP-W), 5% NaOH extract fraction (DNP-OH) and 5% HCl extract fraction (DNP-H). Further the DNP-W was isolated to give six sub-fractions (DNP-W1, DNP-W2, DNP-W3, DNP-W4, DNP-W5 and DNP-W6) by anion-exchange chromatography. The monosaccharide profile, protein content, uronic acid content, total carbohydrate content, viscosity and molecular weight of nine polysaccharide fractions were analyzed. Both the in vivo and in vitro antitumor activities of nine polysaccharide fractions were evaluated and compared. Results indicated that DNP-W1 and DNP-W3 exhibited high antitumor activities against Sarcoma 180 in vivo and HL-60 in vitro. The results suggested that DNP-W1 and DNP-W3 could be considered as an effective natural antitumor source. (C) 2009 Elsevier Ltd. All rights reserved.