Accessory enzymes influence cellulase hydrolysis of the model substrate and the realistic lignocellulosic biomass
Accessory enzymes influence cellulase hydrolysis of the model substrate and the realistic lignocellulosic biomass
复制标题
辅助酶影响模型底物和实际木质纤维素生物质的纤维素酶水解。
DOI：
10.1016/j.enzmictec.2015.06.020
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发表时间：
2015-11-01
影响因子：
3.4
通讯作者：
Shen, Song
中科院分区：
文献类型：
--
作者：
Sun, Fubao Fuebiol;Hong, Jiapeng;Shen, Song
纤维素酶在正在发展和正在进行的“生物精炼”工业中的潜力为开发高效的纤维素酶混合物提供了巨大的动力。最近的研究表明，所谓的辅酶在有效的酶水解中可以发挥多么重要的作用。在这项研究中，三种最新的Novozymes细胞CTEC纤维素酶制剂(CTEC 1/2/3)在相同的FPA负荷下与水蒸气处理的木质纤维底物和模型物质进行了比较。这些纤维素酶制剂表现出明显不同的水解性，与FPA无关。当重新审视基于FPA的检测时，甚至在滤纸本身上也观察到了这种差异。纤维素酶制剂的比酶活性分析表明，不同的辅酶是不同底物和不同纤维素酶之间酶解差异的主要原因。通过添加β-葡萄糖苷酶、木聚糖酶和裂解多糖单加氧酶AA9，最终证实了纤维素酶制剂中存在的辅助酶的活性作用。本文对辅酶的作用有了新的认识，为合理定制纤维素酶鸡尾酒，实现天然木质纤维素底物的高效转化提供了有益的参考。(C)2015 Elsevier Inc.保留所有权利。
The potential of cellulase enzymes in the developing and ongoing "biorefinery" industry has provided a great motivation to develop an efficient cellulase mixture. Recent work has shown how important the role that the so-called accessory enzymes can play in an effective enzymatic hydrolysis. In this study, three newest Novozymes Cellic CTec cellulase preparations (CTec 1/2/3) were compared to hydrolyze steam pretreated lignocellulosic substrates and model substances at an identical FPA loading. These cellulase preparations were found to display significantly different hydrolytic performances irrelevant with the FPA. And this difference was even observed on the filter paper itself when the FPA based assay was revisited. The analysis of specific enzyme activity in cellulase preparations demonstrated that different accessory enzymes were mainly responsible for the discrepancy of enzymatic hydrolysis between diversified substrates and various cellulases. Such the active role of accessory enzymes present in cellulase preparations was finally verified by supplementation with beta-glucosidase, xylanase and lytic polysaccharide monooxygenases AA9. This paper provides new insights into the role of accessory enzymes, which can further provide a useful reference for the rational customization of cellulase cocktails in order to realize an efficient conversion of natural lignocellulosic substrates. (C) 2015 Elsevier Inc. All rights reserved.