Visualizing synapse formation in arborizing optic axons in vivo:: dynamics and modulation by BDNF
Visualizing synapse formation in arborizing optic axons in vivo:: dynamics and modulation by BDNF
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DOI：
10.1038/nn735
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发表时间：
2001-11-01
影响因子：
25
通讯作者：
Cohen-Cory, S
中科院分区：
文献类型：
--
作者：
Alsina, B;Vu, T;Cohen-Cory, S
轴突乔木形态的动态发育变化可直接反映突触的形成、稳定和消除。我们使用双色成像研究，在活的，发育中的动物，轴突分支和突触形成之间的关系，在单细胞水平，并检查参与脑源性神经营养因子（BDNF）在突触发生。绿色荧光蛋白（GFP）标记的小突触蛋白II作为一个标记，可视化在个别荧光标记的非洲爪蟾视神经轴突的突触位点。延时共聚焦显微镜显示，虽然大多数突触保持稳定，突触也形成和消除轴突分支，并增加其复杂性。大多数新分支起源于GFP标记的突触部位。增加BDNF水平显著增加轴突分支和突触数量，BDNF增加每个轴突末端的突触数量。在真实的时间内可视化中枢突触的能力提供了关于突触发生的动力学机制的见解，并揭示了BDNF作为体内突触发生的调节剂。
Dynamic developmental changes in axon arbor morphology may directly reflect the formation, stabilization and elimination of synapses. We used dual-color imaging to study, in the live, developing animal, the relationship between axon arborization and synapse formation at the single cell level, and to examine the participation of brain-derived neurotrophic factor (BDNF) in synaptogenesis. Green fluorescent protein (GFP)-tagged synaptobrevin II served as a marker to visualize synaptic sites in individual fluorescently labeled Xenopus optic axons. Time-lapse confocal microscopy revealed that although most synapses remain stable, synapses are also formed and eliminated as axons branch and increase their complexity. Most new branches originated at GFP-labeled synaptic sites. Increasing BDNF levels significantly increased both axon arborization and synapse number, with BDNF increasing synapse number per axon terminal. The ability to visualize central synapses in real time provides insights about the dynamic mechanisms underlying synaptogenesis, and reveals BDNF as a modulator of synaptogenesis in vivo.