ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development
ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development
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DOI：
10.1111/jipb.12332
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发表时间：
2015-12-01
影响因子：
11.4
通讯作者：
Cardarelli, Maura
中科院分区：
文献类型：
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作者：
Cecchetti, Valentina;Brunetti, Patrizia;Cardarelli, Maura
拟南芥生长素转运蛋白ABCB 1/PGP 1和ABCB 19/PGP 19双突变体abcb 1 abcb 19在雄蕊伸长、花药开裂和花粉成熟过程中发生了改变。为了评估这些转运蛋白对雄蕊发育的贡献，我们对abcb 1和abcb 19单突变体花进行了表型、组织学分析和原位杂交。我们发现，花粉成熟和花药开裂是早熟的abcb 1，但在abcb 19突变体。因此，只在abcb 1花药中改变药室内壁木质化。abcb 1和abcb 1abcb 19雄蕊的早期发育也发生了改变，具有异步的药室和多层绒毡层。DAPI染色结果表明，在abcb 1 abcb 19花药室中，减数分裂的时间是不同步的，而亚历山大染色结果表明，只有一小部分花粉粒是不能生活的。在协议中，TAM（延迟不同步减数分裂），以及BAM 2（几乎任何分生组织）-参与绒毡层细胞发育-在abcb 1 abcb 19年轻的花蕾中过表达。相应地，ABCB 1和ABCB 19 mRNA定位支持观察到的表型abcb 1和abcb 1 abcb 19突变花药。总之，我们提供的证据表明，生长素运输在早期和晚期雄蕊发育中起着重要的作用：ABCB 1在花药发育过程中起着主要作用，而ABCB 19具有协同作用。
Arabidopsis abcb1 abcb19 double mutants defective in the auxin transporters ABCB1/PGP1 and ABCB19/PGP19 are altered in stamen elongation, anther dehiscence and pollen maturation. To assess the contribution of these transporters to stamen development we performed phenotypic, histological analyses, and in situ hybridizations on abcb1 and abcb19 single mutant flowers. We found that pollen maturation and anther dehiscence are precocious in the abcb1 but not in the abcb19 mutant. Accordingly, endothecium lignification is altered only in abcb1 anthers. Both abcb1 and abcb1 abcb19 stamens also show altered early development, with asynchronous anther locules and a multilayer tapetum. DAPI staining showed that the timing of meiosis is asynchronous in abcb1 abcb19 anther locules, while only a small percentage of pollen grains are nonviable according to Alexander's staining. In agreement, TAM (TARDY ASYNCHRONOUS MEIOSIS), as well as BAM2 (BARELY ANY MERISTEM)-involved in tapetal cell development-are overexpressed in abcb1 abcb19 young flower buds. Correspondingly, ABCB1 and ABCB19 mRNA localization supports the observed phenotypes of abcb1 and abcb1 abcb19 mutant anthers. In conclusion, we provide evidence that auxin transport plays a significant role both in early and late stamen development: ABCB1 plays a major role during anther development, while ABCB19 has a synergistic role.