Identification of the earliest prethymic bipotent T/NK progenitor in murine fetal liver
Identification of the earliest prethymic bipotent T/NK progenitor in murine fetal liver
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DOI：
10.1182/blood.v99.2.463
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发表时间：
2002-01-15
期刊：
影响因子：
20.3
通讯作者：
Cumano, A
中科院分区：
文献类型：
--
作者：
Douagi, I;Colucci, F;Cumano, A
本文介绍了胎儿肝脏中新型细胞种群（B220（LO）C-KIT（+）CD19（ - ））的分离，该细胞群体代表该器官中T细胞前体的70％。有趣的是，这些前体显示了二元性T细胞和天然杀伤细胞（NK）限制的重构潜力，但在体内和体外都完全缺乏B和红细胞分化能力。此外，不仅成熟的T细胞受体（TCR）字母（+）外周T细胞，还在恢复的小鼠中产生了tcr-gammadelta（+）和tcralphabeta（+）CD8AlphaalphaalphaalphaalphaalphaalphaalphaAlphaalphaalphaalphaalphaalphaalphaalphaalphaalpha（+）肠道上皮细胞。该人群在胸膜胚胎的胎儿肝脏中的存在表明其起源。 B220（LO）C-KIT（+）CD19（ - ）胎儿肝细胞与胸腺T/NK祖细胞的表型和分化潜力的比较表明，这是到目前为止最不成熟的常见T/NK细胞祖细胞。这些胎儿肝祖细胞可能代表胸腺移民之前的立即发育步骤。 （Blood。2002; 99：463-471）（C）2002年美国血液学学会。
This article describes the isolation of a novel cell population (B220(lo)c-kit(+)CD19(-)) in the fetal liver that represents 70% of T-cell precursors in this organ. Interestingly, these precursors showed a bipotent T-cell and natural killer cell (NK)-restricted reconstitution potential but completely lacked B and erythromyeloid differentiation capacity both in vivo and in vitro. Moreover, not only mature T-cell receptor (TCR)alphabeta(+) peripheral T cells but also TCR-gammadelta(+) and TCRalphabeta(+)CD8alphaalpha(+) intestinal epithelial cells of extrathymic origin were generated in reconstituted mice. The presence of this population in the fetal liver of athymic embryos indicates its prethymic origin. The comparison of the phenotype and differentiation potential of B220(lo)c-kit(+)CD19(-) fetal liver cells with those of thymic T/NK progenitors indicates that this is the most immature common T/NK cell progenitor so far identified. These fetal liver progenitors may represent the immediate developmental step before thymic immigration. (Blood. 2002; 99:463-471) (C) 2002 by The American Society of Hematology.