Biscogniauxia dendrobii sp. nov. and B. petrensis from Dendrobium orchids and the first report of cytotoxicity (towards A549 and K562) of B. petrensis (MFLUCC 14-0151) in vitro
Biscogniauxia dendrobii sp. nov. and B. petrensis from Dendrobium orchids and the first report of cytotoxicity (towards A549 and K562) of B. petrensis (MFLUCC 14-0151) in vitro
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石斛兰
DOI：
10.1016/j.sajb.2020.06.022
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发表时间：
2020-11-01
影响因子：
3.1
通讯作者：
Kang, Ji-Chuan
中科院分区：
文献类型：
--
作者：
Ma, Xiao-Ya;Nontachaiyapoom, Sureeporn;Kang, Ji-Chuan
Biscogniauxia属于Graphostromataceae科，并且在世界范围内主要作为双子叶植物的病原体被发现。本文报道了两个内生菌株，一个是松毛双孢菌（Biscogniauxia dendrobii sp. nov.），另一个是B. petrensis分别从无叶石斛（Dendrobium aphyllum）和铁皮石斛（D. harveyanum分别采自中国西南部和泰国北方。分生孢子梗和分生孢子用于形态学检查。利用ITS、TUB 2、RPB 2和LSU序列数据进行最大似然分析和贝叶斯分析，用于系统发育分析和物种鉴定。基于DNA碱基对差异较大（5.2%）和分生孢子大小的差异，引进了一个新的种-石斛双孢菌（Biscogniauxia dendrobii sp. nov.）。另一个已知种B。petrensis也在这项研究中进行了图解和描述，以便于D.哈维耶南分离物B的乙酸乙酯粗提物。用CCK-8法测得Petrensis对A549和K562有中等和强的细胞毒活性（IC_（50）分别为13.5和3.5 μ g/ml）。这是首次报道石斛属植物内生Biscogniauxia及其对A549和K562的细胞毒性，所有这些都增强了目前对Biscogniauxia属的认识。(c)2020由Elsevier B. V.代表SAAB出版。
Biscogniauxia belongs to the family Graphostromataceae, and species have been found mainly as pathogens in dicotyledonous plants worldwide. Two endophytic strains, Biscogniauxia dendrobii sp. nov. and B. petrensis were isolated from Dendrobium aphyllum and D. harveyanum sampled from southwestern China and northern Thailand respectively. The conidiophores and conidia were used for morphological examination. The maximum likelihood and Bayesian analysis with ITS, TUB2, RPB2 and LSU sequence data were combined for phylogenetic analyses and species identification. Biscogniauxia dendrobii sp. nov. is introduced based on the large DNA base pair differences (5.2%) and size of conidia. Another known species B. petrensis is also illustrated and described in this study to facilitate species identification on D. harveyanum. The ethyl acetate crude extracts of the isolate B. petrensis showed moderate and strong cytotoxic activity against A549 and K562 using the CCK8 assay (IC50 of it 13.5 and 3.5 ug/ml respectively). This is the first report of endophytic Biscogniauxia in Dendrobium species and of their cytotoxicity to A549 and K562, all of which enhances the current knowledge on the genus Biscogniauxia. (c) 2020 Published by Elsevier B.V. on behalf of SAAB.