On-chip cell migration assay using microfluidic channels
On-chip cell migration assay using microfluidic channels
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DOI：
10.1016/j.biomaterials.2007.05.037
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发表时间：
2007-09-01
期刊：
影响因子：
14
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Nie, Fu-Qiang;Yamada, Masumi;Okano, Teruo
细胞迁移在包括胚胎发生、伤口愈合、免疫应答和组织发育在内的各种生物过程中起着至关重要的作用。用于筛选化学引诱剂或化学排斥剂的常规细胞迁移测定通过物理刮擦正常培养表面上的一部分汇合细胞来启动。然而，该方案需要大量的细胞和增加量的试剂。此外，这些方法不适合于放大用于高通量筛选。在这里，我们展示了利用微流体通道的芯片上细胞迁移测定。胰蛋白酶溶液在微流体通道中的层流实现了一部分汇合细胞单层的良好受控的细胞脱离，这可以有效地图案化伤口边缘以模拟体内生物创伤。胰蛋白酶在精确制造的微流体装置中的层流使得能够利用有限量的试剂进行准确和可靠的细胞迁移测定，以促进或抑制细胞迁移。(C)2007爱思唯尔有限公司保留所有权利。
Cell migration plays a crucial role in various biological processes including embryogenesis, wound healing, immune response, and tissue development. Conventional cell migration assays for screening of chemo-attractants or -repellants are initiated by physical scraping of a portion of confluent cells on normal culture surfaces. However, this protocol requires both a large number of cells and an increased amount of reagents. Additionally, these methods are not suitable for scaling-up for high-throughput screening. Here, we show on-chip cell migration assay utilizing microfluidic channels. Laminar flow of trypsin solution in microfluidic channels achieved well-in controlled cell detachment of a portion of confluent cell monolayers, which could effectively pattern wound edges to mimic biological wounding in vivo. Trypsin laminar flow in precisely fabricated microfluidic devices enables accurate and reliable cell migration assay with limited amounts of reagents to either promote or inhibit cell migration. (C) 2007 Elsevier Ltd. All rights reserved.