Analysis of complex particle mixtures by asymmetrical flow field-flow fractionation coupled to inductively coupled plasma time-of-flight mass spectrometry
Analysis of complex particle mixtures by asymmetrical flow field-flow fractionation coupled to inductively coupled plasma time-of-flight mass spectrometry
复制标题
通过不对称流场流分级与电感耦合等离子体飞行时间质谱分析复杂颗粒混合物
DOI：
10.1016/j.chroma.2021.461981
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发表时间：
2021
影响因子：
4.1
通讯作者：
Kraus, Tobias
中科院分区：
文献类型：
--
作者：
Meili-Borovinskaya, Olga;Meier, Florian;Drexel, Roland;Baalousha, Mohammed;Flamigni, Luca;Hegetschweiler, Andreas;Kraus, Tobias
非对称流场-流分馏（AF4）与电感耦合等离子体质谱（ICP-MS）联用已被广泛应用于金属颗粒的表征。本研究证明了AF4与icp -飞行时间质谱（ICP-TOFMS）在标准和单颗粒模式下的耦合优势，可以确定复合颗粒的大小分布、元素组成和数量浓度。耦合系统被用来表征两种复杂的粒子混合物。第一种混合物由从具有不同元素组成的两种尺寸的微合金钢中提取的颗粒组成。第二种混合物由从土壤中提取的颗粒和各种工程纳米颗粒（ENPs）组成。单个微合金钢颗粒的等效水动力尺寸比单颗粒(sp)-ICP-TOFMS测定的尺寸大6倍。较大的AF4尺寸归因于表面涂层的存在，这在sp-ICP-TOFMS测定的芯尺寸中没有反映出来。两个粒子群由于其广泛的尺寸分布而无法被AF4分离，但通过sp-ICP-TOFMS利用其独特的元素特征进行了分离。土壤悬浮液的多角度光散射和ICP-TOFMS信号随ENP浓度的增加而增加。然而，只有通过ICP-TOFMS进行全元素筛选和单颗粒指纹识别，才能将这种增加归因于天然颗粒提取效率的提高，并消除错误结论的风险。在这项研究中，我们描述了如何将AF4偶联到ICP-TOFMS中来研究含有有机化合物的无机颗粒的复杂样品。
Asymmetrical flow field-flow fractionation (AF4) hyphenated with inductively coupled plasma-mass spectrometry (ICP-MS) has been widely used to characterize metal containing particles. This study demonstrates the advantages of coupling AF4 with ICP-time-of-flight mass spectrometry (ICP-TOFMS) in standard and single particle modes to determine size distribution, elemental composition, and number concentration of composite particles. The coupled system was used to characterize two complex particle mixtures. The first mixture consisted of particles extracted from micro-alloyed steels with two size populations of different elemental composition. The second mixture consisted of particles extracted from soil spiked with various engineered nanoparticles (ENPs). The equivalent hydrodynamic sizes of individual micro-alloyed steel particles were up to 6 times larger than the sizes determined by single particle (sp)-ICP-TOFMS. The larger AF4 sizes were attributed to the presence of a surface coating, which is not reflected in the core size determined by sp-ICP-TOFMS. Two particle populations could not be separated by AF4 due to their broad size distributions but were resolved by sp-ICP-TOFMS using their unique elemental signatures. Multi-angle light scattering and ICP-TOFMS signals of soil suspensions increased with the spiked ENP concentrations. However, only after conducting full element screening and single particle fingerprinting by ICP-TOFMS could this increase be attributed to enhanced extraction efficiency of natural particles and the risk for false conclusions be eliminated. In this study, we describe how AF4 coupled to ICP-TOFMS can be applied to study complex samples of inorganic particles which contain organic compounds.