Upregulation of uncoupling protein-3 in skeletal muscle during exercise: a potential antioxidant function
Upregulation of uncoupling protein-3 in skeletal muscle during exercise: a potential antioxidant function
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DOI：
10.1016/j.freeradbiomed.2008.09.026
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发表时间：
2009-01-15
影响因子：
7.4
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
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作者：
Jiang, Ning;Zhang, Guizhong;Zhang, Yong
解偶联蛋白-3（UCP 3）的表达已被证明在肌肉收缩反应中显着增加，但UCP 3上调的生理意义仍然难以捉摸。在本研究中，UCP 3 mRNA和蛋白的表达沿着随着线粒体呼吸功能，活性氧（ROS）的产生，以及在脂肪骨骼肌的抗氧化防御过程中和之后的急性回合长时间的运动。UCP 3 mRNA的表达在45分钟的运动急剧上升，达到7- 8倍以上的静息水平在150分钟。UCP 3蛋白含量的增加表现出潜在的反应，但升高类似于1.9倍，在120分钟的运动。运动后24 h，UCP 3 mRNA和蛋白水平逐渐恢复到实验水平。运动过程中线粒体ROS的产生逐渐增加。然而，ROS在150分钟时显示出急剧下降，尽管它们的水平在恢复期间仍然高出几倍。线粒体状态4呼吸速率分别增加46%和58%（p
Uncoupling protein-3 (UCP3) expression has been shown to increase dramatically in response to muscular contraction, but the physiological significance of UCP3 upregulation is still elusive. In this study, UCP3 mRNA and protein expression were investigated along with mitochondrial respiratory function, reactive oxygen species (ROS) generation, and antioxidant defense in Fat skeletal Muscle during and after an acute bout of prolonged exercise. UCP3 mRNA expression was elevated sharply at 45 min of exercise, reaching 7- to 8-fold above resting level at 150 min. The increase in UCP3 protein content showed a latent response but was elevated similar to 1.9-fold at 120 min of exercise. Both UCP3 mRNA and UCP3 protein gradually returned to testing levels 24 h postexercise. Mitochondrial ROS production was progressively increased during exercise. However, ROS showed a dramatic drop at 150 min although their levels remained severalfold higher during the recovery. Mitochondrial State 4 respiration rate was increased by 46 and 58% (p