Expression of pigment-epithelium-derived factor during kidney development and aging
Expression of pigment-epithelium-derived factor during kidney development and aging
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DOI：
10.1007/s00441-007-0420-8
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发表时间：
2007-08-01
影响因子：
3.6
通讯作者：
Kloth, Sabine
中科院分区：
文献类型：
--
作者：
Pina, Ana Luisa;Kubitza, Marion;Kloth, Sabine
内皮细胞生长的抑制和刺激因子在器官生长发育过程中对血管形成的协调是必不可少的。在成人肾脏中，色素上皮衍生因子(PEDF)是血管生成的主要抑制因子之一。我们分析了PEDF在肾脏发育和衰老的不同阶段的表达和分布，特别是功能性肾小球的形成。我们发现，随着年龄的增长，肾脏中PEDF基因的表达和蛋白水平显著增加。我们已经在研究的所有发育阶段的间质和内皮细胞中检测到PEDF，在与肾单位和集合管的发育有关的新血管形成区的所有区域，以及在成人肾脏的成熟足细胞中都检测到了PEDF。我们的结果首次表明PEDF在肾脏出生后早期发育中起重要作用，它可能与肾小球功能的成熟和这些细胞形成的滤过屏障有关，并可能在肾脏发育过程中作为一种抗血管生成调节剂。
Inhibitors and stimulators of endothelial cell growth are essential for the coordination of blood vessel formation during organ growth and development. In the adult kidney, one of the major inhibitors of angiogenesis is pigment-epithelium-derived factor (PEDF). We have analyzed the expression and distribution of PEDF during various stages of renal development and aging with particular emphasis on the formation of functional glomeruli. We show that PEDF gene expression and protein levels in the kidney significantly increase with age. We have detected PEDF in the mesenchyme and endothelial cells at all developmental stages studied, in all regions of the nephrogenic zone in which the formation of new blood vessels is associated with the development of nephrons and collecting ducts, and in mature podocytes in the adult kidney. Our results are the first to suggest that PEDF is important in early renal postnatal development, that it could be relevant to the maturation of glomerular function and the filtration barrier formed by these cells, and that it may serve as an anti-angiogenic modulator during kidney development.