Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers
Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers
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DOI：
10.1111/cei.12325
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发表时间：
2014-07-01
影响因子：
4.6
通讯作者：
Rogers, T. J.
中科院分区：
文献类型：
--
作者：
Chatila, W. M.;Criner, G. J.;Rogers, T. J.
Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal regulatory T cell (Treg) response and increases in T helper type 1 (Th1) and Th17 cell responses. It is unclear if dysregulation of microRNAs (miRNA) within Treg cells contributes to the abnormal inflammatory response in COPD. In this study, we aimed to compare the miRNA profile of COPD Treg cells with that of healthy controls and to explore the function of differentially expressed miRNAs. We first obtained Treg and T effector cells (Teff) from peripheral blood of non-smokers, unaffected current smokers and COPD current smokers. Then, we assessed their miRNA expression by microarray analysis followed by real-time reverse transcription-polymerase chain reaction (RT-PCR) validation of particular miRNAs. Six and 96 miRNAs were expressed differentially in COPD Treg cells versusTreg cells of healthy non-smokers and healthy smokers, whereas no differences were found in miRNA expression in Teff cells. We found that miR-199a-5p was repressed by approximately fourfold in Treg cells of COPD patients compared to healthy smokers (P
