Anti-catabolic effect of caffeic acid phenethyl ester, an active component of honeybee propolis on bone loss in ovariectomized mice: a micro-computed tomography study and histological analysis
Anti-catabolic effect of caffeic acid phenethyl ester, an active component of honeybee propolis on bone loss in ovariectomized mice: a micro-computed tomography study and histological analysis
复制标题
蜂胶活性成分咖啡酸苯乙酯对卵巢切除小鼠骨质流失的抗分解代谢作用：微型计算机断层扫描研究和组织学分析
DOI：
10.3760/cma.j.issn.0366-6999.20141399
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发表时间：
2014-11-20
影响因子：
6.1
通讯作者：
Wei Xiaochun
中科院分区：
文献类型：
--
作者：
Duan Wangping;Wang Qing;Wei Xiaochun
研究背景骨质疏松症（Osteoporosis，OP）是一种常见的骨疾病，严重影响人们的生活质量。有效的治疗是必要的，以打击双方的损失和骨折的骨。咖啡酸苯乙酯（CAPE）是一种从蜜蜂幼虫中提取的天然化合物，具有抑制破骨细胞生成和骨吸收的作用。因此，本研究旨在探讨CAPE对骨质疏松症小鼠骨丢失的影响，使用显微计算机断层扫描（CT）和histologics.Methods制备18只小鼠，平均分为三组。假手术+PBS组中的6只小鼠不进行卵巢切除术，并在治疗期间腹膜内注射PBS。12只小鼠切除卵巢（OVX）诱导OP，OVX+CAPE组6只小鼠切除卵巢后腹腔注射CAPE 0.5mg/kg，每周2次，共4周。OVX+PBS组中的另外6只OVX小鼠用PBS处理。卵巢切除后4周处死所有小鼠。切除双侧胫骨进行显微CT扫描和组织学分析。采用Mann-Whitney U检验进行统计学分析。与假手术+PBS组相比，OVX+PBS组小鼠骨密度显著降低（BMD，P
Background Osteoporosis (OP) is a common bone disease, which adversely affects life quality. Effective treatments are necessary to combat both the loss and fracture of bone. Recent studies indicated that caffeic acid phenethyl ester (CAPE) is a natural chemical compound from honeybee propolis which is capable of attenuating osteoclastogenesis and bone resorption. Therefore, this study aimed to investigate the effect of CAPE on bone loss in OP mice using micro-computed tomography (CT) and histology.Methods Eighteen mice were prepared and evenly divided into three groups. The six mice in the sham+PBS group did not undergo ovariectomy and were intraperitoneally injected with PBS during the curing period. Twelve mice were ovariectomized (OVX) to induce OP. Six of them in the OVX+CAPE group were intraperitoneally injected with 0.5 mg/kg CAPE twice per week for 4 weeks after ovariectomy. The other six OVX mice in OVX+PBS group were treated with PBS. All the mice were sacrificed 4 weeks after ovariectomy. The tibias were bilaterally excised for micro-CT scan and histological analysis. The Mann-Whitney U test was used to test the statistical differences among groups.Results Bone loss occurred in OVX mice. Compared with the sham+PBS group, mice in the OVX+PBS group exhibited a significant decrease in bone mineral density (BMD, P