INTERACTION OF INFLUENZA-A VIRUS WITH HUMAN PERIPHERAL-BLOOD LYMPHOCYTES
INTERACTION OF INFLUENZA-A VIRUS WITH HUMAN PERIPHERAL-BLOOD LYMPHOCYTES
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DOI：
10.1128/iai.25.2.749-756.1979
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
HAZLEMAN, BL
中科院分区：
文献类型：
--
作者：
BROWNSON, JM;MAHY, BWJ;HAZLEMAN, BL
在植物血凝素、刀豆球蛋白 A 或商陆有丝分裂原存在下培养的外周血淋巴细胞在体外暴露于甲型流感病毒。尽管淋巴细胞培养物没有产生感染性病毒，但检测到几种病毒特异性蛋白质的合成，包括核蛋白、膜蛋白和非结构1蛋白。只有丝裂原转化细胞的亚群才能支持病毒蛋白的合成。甲型流感病毒与正常个体和类风湿性关节炎患者的淋巴细胞相互作用的比较表明，无论淋巴细胞的来源如何，都会以相似的数量产生相同范围的病毒特异性蛋白。
Peripheral blood lymphocytes cultured in the presence of phytohemagglutinin, concanavalin A or pokeweed mitogen were exposed in vitro to influenza A virus. The synthesis of several virus-specific proteins, including the nucleoprotein, membrane protein and non-structural 1 protein were detected although no infectious virus was produced by the lymphocyte cultures. Only a subpopulation of mitogen-transformed cells would support virus protein synthesis. A comparison of the interactions of influenza A virus with lymphocytes from normal individuals and from rheumatoid arthritis patients showed that the same range of virus-specific proteins were made in similar quantities regardless of the source of lymphocytes.