The Shape of Sound: a Geometric Morphometrics Approach to Laryngeal Functional Morphology
The Shape of Sound: a Geometric Morphometrics Approach to Laryngeal Functional Morphology
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DOI：
10.1007/s10914-019-09466-9
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发表时间：
2020-09-01
影响因子：
1.9
通讯作者：
Riede, Tobias
中科院分区：
文献类型：
--
作者：
Borgard, Heather L.;Baab, Karen;Riede, Tobias
动物发声的多样化在行为进化和物种形成中发挥着关键作用。发声器官形态是声学变化的重要来源，但其尺寸小、形状复杂且缺乏同源标志，给比较分析带来了重大挑战。在这里，我们使用基于几何同源地标的几何形态测量方法来量化代表三个科的四个啮齿动物属的喉软骨的形状变化。通过放置在结构边缘和主要表面上的不同数量的三维标志来量化对比增强显微 CT 图像重建的喉软骨。在统计分析之前使用广义普罗克拉斯特分析来叠加地标集。成对 Procrustes 距离之间的相关性用于确定充分表征形状变化所需的最小数量的标志。我们发现这五个物种在形态空间中占据不同的位置，其变异部分可以通过系统发育、体型和发声机制的差异来解释。我们的研究结果为量化发声器官形态对声学多样化的贡献奠定了基础。
Diversification of animal vocalizations plays a key role in behavioral evolution and speciation. Vocal organ morphology represents an important source of acoustic variation, yet its small size, complex shape, and absence of homologous landmarks pose major challenges to comparative analyses. Here, we use a geometric morphometric approach based on geometrically homologous landmarks to quantify shape variation of laryngeal cartilages of four rodent genera representing three families. Reconstructed cartilages of the larynx from contrast-enhanced micro-CT images were quantified by variable numbers of three-dimensional landmarks placed on structural margins and major surfaces. Landmark sets were superimposed using generalized Procrustes analysis prior to statistical analysis. Correlations among pairwise Procrustes distances were used to identify the minimum number of landmarks necessary to fully characterize shape variation. We found that the five species occupy distinct positions in morphospace, with variation explained in part by phylogeny, body size, and differences in vocal production mechanisms. Our findings provide a foundation for quantifying the contribution of vocal organ morphology to acoustic diversification.